CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
4.4.2.1 Basic method settings
The basic settings allow you to control the %B gradient mix,
flow rate, solvents, and run length. These settings are part of
the MAIN WINDOW [4.3.1]. These settings are the most
frequently changed settings when developing or improving
purification methods.
The %B gradient mix and run length are shown on the plot
area. Refer to the DEFINING A GRADIENT [4.4.5] topic for more
details. The other settings are:
• Flow Rate — When using the Isco default column
methods, the flow rate is automatically set to the
optimum flow rate of the column. If using a non-Isco
column, refer to the manufacturer’s column
documentation to determine the best flow rate for the
column. Note that if the column is not detected by the
module, the operating pressure is limited to 50 psi.
• Solvent A and B — Select the desired solvents from the
lists. The solvents are those defined by the
CONFIGURATION [4.3.10] window.
4.4.2.2 Advanced method settings
The METHOD EDITOR [4.3.2] window gives you access to
advanced settings such as equilibration volume, initial waste,
air purge, peak detection, and peak collection mode.
Equilibration Volume Column equilibration is optional, but
you may find that separations are more effective if the column
is equilibrated before injecting the sample. The recommended
equilibration volume is at least three column volumes of the
starting mobile phase.
RediSep Rf Silica gel column volumes are:
• RediSep Rf 80 g = 125 mL
• RediSep Rf 120 g = 192 mL
• RediSep Rf 220 g = 334 mL
• RediSep Rf 330 g = 443 mL
• RediSep Rf 750 g = 1.2 L
• RediSep Rf 1.5 kg = 2.4 L
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