CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
4.4.2.1 Basic method settings<br />
The basic settings allow you to control the %B gradient mix,<br />
flow rate, solvents, and run length. These settings are part of<br />
the MAIN WINDOW [4.3.1]. These settings are the most<br />
frequently changed settings when developing or improving<br />
purification methods.<br />
The %B gradient mix and run length are shown on the plot<br />
area. Refer to the DEFINING A GRADIENT [4.4.5] topic for more<br />
details. The other settings are:<br />
• Flow Rate — When using the <strong>Isco</strong> default column<br />
methods, the flow rate is automatically set to the<br />
optimum flow rate of the column. If using a non-<strong>Isco</strong><br />
column, refer to the manufacturer’s column<br />
documentation to determine the best flow rate for the<br />
column. Note that if the column is not detected by the<br />
module, the operating pressure is limited to 50 psi.<br />
• Solvent A and B — Select the desired solvents from the<br />
lists. The solvents are those defined by the<br />
CONFIGURATION [4.3.10] window.<br />
4.4.2.2 Advanced method settings<br />
The METHOD EDITOR [4.3.2] window gives you access to<br />
advanced settings such as equilibration volume, initial waste,<br />
air purge, peak detection, and peak collection mode.<br />
Equilibration Volume Column equilibration is optional, but<br />
you may find that separations are more effective if the column<br />
is equilibrated before injecting the sample. The recommended<br />
equilibration volume is at least three column volumes of the<br />
starting mobile phase.<br />
RediSep Rf Silica gel column volumes are:<br />
• RediSep Rf 80 g = 125 mL<br />
• RediSep Rf 120 g = 192 mL<br />
• RediSep Rf 220 g = 334 mL<br />
• RediSep Rf 330 g = 443 mL<br />
• RediSep Rf 750 g = 1.2 L<br />
• RediSep Rf 1.5 kg = 2.4 L<br />
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