CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
If you will transfer the sample mixture directly from a
container, attach the tubing to the pump inlet. Next,
place the weighted end into the mixture container.
c. Place the On/Off (I/O) switch in the On position.
Adjust the pump speed using the control knob.
d. When the desired sample volume has been loaded,
place the On/Off (I/O) switch in the Off position.
e. Some of the sample mixture will remain in the tubing
before the automated injection valve. In most
applications, pump a chase solution to force the
sample mixture through the injection valve. To do so,
repeat steps b through d using a suitable chase
solvent.
You may allow the module to proceed with the run while
monitoring the progress on the PeakTrak Main window. If
desired, gradient parameters may be modified during the run.
(See REAL-TIME GRADIENT EDITING [4.4.6].)
As the run progresses, the absorbance trace is drawn on the
Gradient Plot area. The purification continues until the end of
the run defined by the run length setting.
Note: Solvent may be under pressure. Do not remove a
column or solid load cartridge until the pressure gauge on the
touch screen to return to 0 psi.
Note: After use, pump clean solvent to remove sample
residue.
See Also:
REDISEP RF COLUMN SELECTION GUIDE [ON-LINE]
SOLVENT SELECTION TABLE [ON-LINE]
EDITING A METHOD [4.4.2]
EDITING A DEFAULT METHOD [4.4.4]
BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]
4.4.2 Editing a method
Editing a method allows you to tailor the operation of the
CombiFlash Torrent module to best separate or purify the
compounds of interest.
PeakTrak has two types of method settings — basic and
advanced.
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