CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
If you selected Solid (Pause), the module equilibrates<br />
the column with the starting %B and then waits while<br />
you prepare the solid sample load cartridge. Place the<br />
cartridge on the module and click OK to continue.<br />
If you selected Solid, the module equilibrates the<br />
column and immediately starts the separation.<br />
If you selected None (on column), the module skips the<br />
equilibration and immediately starts the separation.<br />
If you selected Liquid, the module equilibrates the<br />
column and then waits. When instructed, perform either<br />
a Syringe Injection or use the Sample Load Pump:<br />
Syringe Injection:<br />
a. Connect the Liquid Injection Accessory from the<br />
accessory kit to the Sample Injection Port.<br />
b. Place the syringe on top of the Liquid Injection<br />
Accessory valve.<br />
c. Open the valve by turning the handle so it is in line<br />
with the tubing.<br />
d. Inject the liquid sample mixture.<br />
e. Close the valve by turning the handle so that it is<br />
perpendicular to the tubing. Remove the syringe.<br />
f. Inject a chase solvent by connecting a syringe loaded<br />
with a chase solvent to the Liquid Injection<br />
accessory’s luer fitting.<br />
g. Open the valve by turning the handle so it is in line<br />
with the tubing.<br />
h. Inject at least 10 mL of the chase solvent.<br />
i. Close the valve by turning the handle so that it is<br />
perpendicular to the tubing. Remove the syringe.<br />
j. Click the OK button to continue the run.<br />
Sample Load Pump:<br />
a. Connect the outlet tubing from the Sample Load<br />
pump to the Torrent module’s Sample Injection Port.<br />
b. Prepare the Sample Load pump to receive the sample:<br />
If the sample mixture will be transferred to the pump,<br />
insert the empty cartridge body onto the pump inlet.<br />
Then, fill the cartridge with a measured mixture<br />
volume.<br />
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