CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
equilibration and then wait while you prepare the
cartridge. After you have placed the cartridge on the
module, click OK to continue with the run.
• Solid – Select this option if you have already
prepared the sample, placed it into a solid sample
load cartridge, and loaded the cartridge on the
module. The module will automatically proceed until
the end of the programmed run length.
• Liquid – Select this option if you have prepared a
liquid sample and plan to manually inject it or pump
into the injection port after column equilibration.
• None (on column) – Select this option if you have
preloaded the sample on the column. The module will
skip column equilibration so that the sample will not
be flushed from the column before the run.
5.Review or update the Start Rack and Start Tube settings.
6.Review the Minimum Run Requirements. PeakTrak will
report the estimated solvent volumes, expected waste,
collection tube usage, etc. You can use this information to
verify that there will be enough solvent to complete the
run, the waste volume does not exceed the collection
container’s capacity, and whether or not more tubes will be
required during the run.
Note: The module estimates the solvent volumes by
monitoring the solvent level in the container and the
known usage rate. The module continues to refine this
estimate during operation. See also: ABOUT SOLVENT
LEVEL DETECTION [ON-LINE].
Note: If the waste level sensing tube is inserted
correctly into the waste container, the module
automatically will suspend operation before an overflow
condition might exist. To prevent the run from being
suspended before completing the run, ensure that the
container will hold the expected waste volume.
7.Click OK and the run begins. The module responds
according to the sample Loading Type you selected in
step 4.
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