CombiFlash Torrent® User Manual - Isco

CombiFlash Torrent® User Manual
•MONITORING THE WAVELENGTH RATIO [4.4.7]
•BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]
•VIEWING RUNS [4.4.9]
•MANUAL CONTROL OF THE COMBIFLASH TORRENT
MODULE [4.4.10]
•SETTING THE VAPOR LIMIT [4.4.11]
4.4.1 Starting a run using default settings
After the CombiFlash Torrent module has been automatically
primed (See AUTO PRIME/VALVE WASH [4.3.7]) and a RediSep Rf
column has been inserted, the module is ready to perform a
run using the default settings. These default settings are
preset to the most common configurations used by chemists,
and have been optimized for the use of RediSep Rf columns.
The default settings will:
• collect all fluid in the fraction collector rack
• differentiate between UV (254 nm) vs. non-UV absorbing
fluid and place the fluids into separate tubes
• cut UV absorbing peaks based on slope detection or level
threshold.
To start the run from the PeakTrak Main window:
1. Enter a sample name. If you choose not to, PeakTrak will
enter the date and time as the sample name when you start
the run.
2.Review the PeakTrak Main window settings. If you want to
change any settings, refer to the Editing a Method on-line
help topic.
3.Click the Play button.
4.Select the sample Loading Type from the list. The Loading
Type should support the sample injection technique you
intend to use (see SAMPLE INJECTION METHODS [ON-LINE]).
Possible sample loading types are:
• Solid (Pause) – Select this option if you intend place
the sample into a solid sample load cartridge, but
have not yet prepared the sample. When you click the
run button, the module will perform a column
4-34