CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
CombiFlash Torrent® User Manual - Isco
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<strong>CombiFlash</strong> Torrent® <strong>User</strong> <strong>Manual</strong><br />
•MONITORING THE WAVELENGTH RATIO [4.4.7]<br />
•BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]<br />
•VIEWING RUNS [4.4.9]<br />
•MANUAL CONTROL OF THE COMBIFLASH TORRENT<br />
MODULE [4.4.10]<br />
•SETTING THE VAPOR LIMIT [4.4.11]<br />
4.4.1 Starting a run using default settings<br />
After the <strong>CombiFlash</strong> Torrent module has been automatically<br />
primed (See AUTO PRIME/VALVE WASH [4.3.7]) and a RediSep Rf<br />
column has been inserted, the module is ready to perform a<br />
run using the default settings. These default settings are<br />
preset to the most common configurations used by chemists,<br />
and have been optimized for the use of RediSep Rf columns.<br />
The default settings will:<br />
• collect all fluid in the fraction collector rack<br />
• differentiate between UV (254 nm) vs. non-UV absorbing<br />
fluid and place the fluids into separate tubes<br />
• cut UV absorbing peaks based on slope detection or level<br />
threshold.<br />
To start the run from the PeakTrak Main window:<br />
1. Enter a sample name. If you choose not to, PeakTrak will<br />
enter the date and time as the sample name when you start<br />
the run.<br />
2.Review the PeakTrak Main window settings. If you want to<br />
change any settings, refer to the Editing a Method on-line<br />
help topic.<br />
3.Click the Play button.<br />
4.Select the sample Loading Type from the list. The Loading<br />
Type should support the sample injection technique you<br />
intend to use (see SAMPLE INJECTION METHODS [ON-LINE]).<br />
Possible sample loading types are:<br />
• Solid (Pause) – Select this option if you intend place<br />
the sample into a solid sample load cartridge, but<br />
have not yet prepared the sample. When you click the<br />
run button, the module will perform a column<br />
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