CombiFlash Torrent® User Manual - Isco

Section 4 User Help Reference
Note: If both Slope and Threshold peak detection
methods are checked, PeakTrak considers a peak to
be present when any one condition is met. This logical
OR operator means that PeakTrak will cut a peak
when either the Slope condition is true, or when the
Threshold condition is true.
• All Wavelength Detection — Enable this option to
detect peaks within a user-selected range of
wavelengths. When enabled, click the Details button to
configure additional settings. These settings include the
slope-based and peak width options described in 1 and
2 above. Additionally, you can type or select the
minimum and maximum wavelengths limits in
nanometers.
• External Detector — Enable this option to use a 0 to 1
volt analog signal from an external detector. When
enabled, click the Details button to configure additional
settings. These settings include the slope-based and
peak width options described in 1 and 2 above.
PeakTrak will use its internal algorithms to detect and
cut peaks based on the analog input signal. Refer to the
External Detector instruction sheet for electrical and
plumbing requirements.
• Show Ratio — If using two of the above peak detection
options, check this option to display a ratio of the
selected wavelengths. The ratio trace is often a useful
indicator of purity. Refer to the MONITORING THE
WAVELENGTH RATIO [4.4.7] topic for more details on this
feature.
4.3.3 Gradient Optimizer
Use this window to automatically generate a gradient curve
from the results of two TLC plates. Follow the on-screen
instructions, then enter valid TLC data in the Gradient
Optimizer window. Click the OK button and the Method
Editor window will display the new gradient curve.
When using the Gradient Optimizer, PeakTrak may insert an
isocratic hold into the generated gradient. Furthermore,
PeakTrak may automatically extend the isocratic hold during
the run to increase peak resolution.
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