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6100CR Refrigerated Sampler - Isco

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<strong>6100CR</strong> <strong>Refrigerated</strong> <strong>Sampler</strong><br />

Section 2 Programming<br />

After the 50 second interval has passed, the flow valve will<br />

shut off and the following display will appear:<br />

REPEAT CALIBRATION<br />

YES<br />

NO<br />

Followed by (if Yes is selected):<br />

PUT DISCHARGE TUBE<br />

IN GRAD CYLINDER<br />

Before repeating the calibration, measure the flow volume<br />

you have collected in the graduated cylinder. The collected<br />

volume should be between 90 and 110 ml. If it is low,<br />

increase the flow slightly by turning the handle on the<br />

pressure regulator clockwise. If the collected volume is<br />

high, turn the regulator handle counter-clockwise slightly<br />

to decrease the flow.<br />

Select “YES” for the repeat calibration until the collected<br />

volume is between 90 and 110 ml. Then select “NO” to continue<br />

the Setup sequence. Once calibration is successfully<br />

completed, lock the regulator adjustment knob in place by<br />

holding the knob still in your hand and tightening the locking<br />

ring against the bottom of the knob. If a considerable<br />

number of calibration routines have to be run to achieve<br />

proper calibration, the precooler will warm up and not provide<br />

the usual amount of cooling if the first sample is taken<br />

immediately.<br />

3. If you select NO for CALIBRATE FLOW RATE, the following<br />

will appear:<br />

STRAINER PURGE TIME<br />

(XX) SECONDS (0-600)<br />

The value entered for purge time is dependent on several<br />

factors. First is the amount of carryover from sample to<br />

sample that is acceptable. Second is the amount of time<br />

that can be allocated to purging. Third is the length of the<br />

sample line. Tests have indicated that a minimum of seven<br />

volumes are needed to reduce the carryover in the strainer<br />

to 1%. Based on the volume of the strainer, Teledyne <strong>Isco</strong><br />

recommends a setting of from 875 - 1250 ml to purge the<br />

strainer and 60 ml per 25 feet of line from the source to the<br />

sampler.<br />

The flow rate through the strainer “bypass” is somewhat<br />

variable, ranging from 400-500 ml/minute. It can be<br />

checked by pressing Manual Purge in the Standby state<br />

and then selecting “Strainer Purge” and measuring the<br />

amount of liquid collected.<br />

2-4

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