D-Series Syringe Pumps - Isco

D Series Syringe Pumps: Models 65DM, 100DM, 100DX, 260D
Section 2A 65DM, 100DM, 100DX, and 260D Liquid System Connections & Accessories
2A.2.2 Installation Tips • Be sure to keep the tubing as straight as possible at the
end, as this will make it easier to install the ferrules.
• Be sure to cut the ends of the tubing squarely.
• Don’t leave burrs on the ends of the tubing.
• When installing ferrules on the tubing, be sure the
tubing extends beyond the ferrule to allow for proper
crimping.
• If the connection is leaking, retighten fittings.
• Push the tubing completely into the port before tightening
the nut.
• When connections are made to the cylinder cap, the
pressure reading may be affected. If the pressure no
longer reads zero, press ZERO PRESSURE on the front
panel of the controller to readjust.
2A.2.3 Tubing Cutting To prevent possible problems, it is important to squarely cut the
tubing. Square ends will be easier to insert through the ferrule
and will lower the dead volume.
It is recommended that electrochemically machined steel tubing
be used throughout the plumbing system. Electrochemically
machined tubing has flat, burr-free ends for minimum dead
volumes and is free of cutting residues. Pre-cut, electrochemically
machined tubing is available through many chromatographic
supply distributors in assorted lengths.
A somewhat less desirable alternative is to purchase a tubing
cutter designed to handle steel tubing.
For quick fixes, the tubing may also be cut by hand with the following
procedure. A jewelers file, goggles, and two pairs of pliers
are necessary for this operation.
To cut the tubing by hand
1. Wear goggles. Using a fine jewelers file, score the tubing
around its entire circumference.
2. Secure the tubing with pliers on each side of the score line
leaving approximately 1.5 mm between each set of pliers
and the score line. Care must be taken not to squeeze the
tubing too tightly as that will flatten or deform the exterior
of the tubing.
3. With the pliers, bend the tubing back and forth to cause
cracking at the score line. The bending should be done in
two places to reduce the chance of squashing the tubing.
4. It may be necessary to deburr the outside of the tubing
ends with the file. Make sure the tubing ends are clean and
the inner bore is clear before installing the cut tube.
Note
It is often impossible to remove a burr that blocks the inner
bore.
2A-3