D-Series Syringe Pumps - Isco

D Series Syringe Pumps
Section 9 Modifier Addition
8. When A pressure is established, the message “HOLD:
PRESS RUN” will be displayed.
9. Open the extractor inlet valves.
10. The message “EQUILIBRATE” will be displayed on the
controller screen. If the controller does not display
“EQUILIBRATE,” press the RUN key. While this message
is displayed, no modifier will be delivered.
11. Immediately open the extractant outlet valve(s).
Note
Flow MUST be established through the system at or before the
time “EQUILIBRATE” is displayed. To properly equilibrate the
pumps, they must be delivering fluid.
12. Once the message “Running” is displayed, modifier will be
delivered at the selected rate.
To refill pump B (the
modifier pump)
1. Press the STOP key.
2. Close the outlet valves of both pumps.
3. Take the pumps out of modifier mode by pressing softkey B
under the words “MODIFIER”. The word ON will change
to OFF above the modifier.
4. Open the inlet valve of pump B.
5. Press the REFILL key, and press softkey B to designate
Pump B.
6. After pump B has filled, press the CONST FLOW key and
set the desired purging flow rate. Press the RUN key and
deliver 5-10 ml of modifier back through the inlet valve.
This ensures that all air is purged from the pump.
7. Press the red STOP key on the controller front panel keypad.
Then press softkey B.
To start modifier 1. Return to constant pressure mode by pressing softkey D,
“SELECT PUMP,” and then softkey A.
2. Place the pump in modifier mode by pressing softkey B
under the words “MODIFIER.” The word OFF will change
to ON above “MODIFIER.”
3. Open the outlet valves of both pumps if they are not open.
4. Press the RUN key on the controller front panel keypad.
5. When A pressure is established, the message “HOLD:
PRESS RUN” will be displayed.
6. Open the extractor inlet valves.
7. The message “EQUILIBRATE” will be displayed on the
controller screen. If the controller does not display
“EQUILIBRATE,” press the RUN key. While this message
is displayed, no modifier will be delivered.
8. Immediately open the extractant outlet valve(s).
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