D-Series Syringe Pumps - Isco
D-Series Syringe Pumps - Isco
D-Series Syringe Pumps - Isco
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D <strong>Series</strong> <strong>Syringe</strong> <strong>Pumps</strong><br />
Section 9 Modifier Addition<br />
8. When A pressure is established, the message “HOLD:<br />
PRESS RUN” will be displayed.<br />
9. Open the extractor inlet valves.<br />
10. The message “EQUILIBRATE” will be displayed on the<br />
controller screen. If the controller does not display<br />
“EQUILIBRATE,” press the RUN key. While this message<br />
is displayed, no modifier will be delivered.<br />
11. Immediately open the extractant outlet valve(s).<br />
Note<br />
Flow MUST be established through the system at or before the<br />
time “EQUILIBRATE” is displayed. To properly equilibrate the<br />
pumps, they must be delivering fluid.<br />
12. Once the message “Running” is displayed, modifier will be<br />
delivered at the selected rate.<br />
To refill pump B (the<br />
modifier pump)<br />
1. Press the STOP key.<br />
2. Close the outlet valves of both pumps.<br />
3. Take the pumps out of modifier mode by pressing softkey B<br />
under the words “MODIFIER”. The word ON will change<br />
to OFF above the modifier.<br />
4. Open the inlet valve of pump B.<br />
5. Press the REFILL key, and press softkey B to designate<br />
Pump B.<br />
6. After pump B has filled, press the CONST FLOW key and<br />
set the desired purging flow rate. Press the RUN key and<br />
deliver 5-10 ml of modifier back through the inlet valve.<br />
This ensures that all air is purged from the pump.<br />
7. Press the red STOP key on the controller front panel keypad.<br />
Then press softkey B.<br />
To start modifier 1. Return to constant pressure mode by pressing softkey D,<br />
“SELECT PUMP,” and then softkey A.<br />
2. Place the pump in modifier mode by pressing softkey B<br />
under the words “MODIFIER.” The word OFF will change<br />
to ON above “MODIFIER.”<br />
3. Open the outlet valves of both pumps if they are not open.<br />
4. Press the RUN key on the controller front panel keypad.<br />
5. When A pressure is established, the message “HOLD:<br />
PRESS RUN” will be displayed.<br />
6. Open the extractor inlet valves.<br />
7. The message “EQUILIBRATE” will be displayed on the<br />
controller screen. If the controller does not display<br />
“EQUILIBRATE,” press the RUN key. While this message<br />
is displayed, no modifier will be delivered.<br />
8. Immediately open the extractant outlet valve(s).<br />
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