D-Series Syringe Pumps - Isco
D-Series Syringe Pumps - Isco
D-Series Syringe Pumps - Isco
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D <strong>Series</strong> <strong>Syringe</strong> <strong>Pumps</strong><br />
Section 9 Modifier Addition<br />
7. MODIFIER ON will be displayed above softkey B, and %<br />
PUMP will be displayed above softkey C. To designate the<br />
desired modifier concentration, press softkey C. Then use<br />
the numeric keys to enter the desired percentage of modifier.<br />
Press the enter key to save the desired value.<br />
8. Open the outlet valves of both pumps if they are not open.<br />
9. Press the RUN key on the controller front panel keypad.<br />
10. When A pressure is established, the message “HOLD:<br />
PRESS RUN” will be displayed.<br />
11. Open the extractor inlet valves.<br />
12. The message “EQUILIBRATE” will be displayed on the<br />
controller screen. If the controller does not display<br />
“EQUILIBRATE,” press the RUN key. While this message<br />
is displayed, no modifier will be delivered.<br />
13. Immediately open the extractant outlet valve(s).<br />
Note<br />
Flow MUST be established through the system at or before the<br />
time “EQUILIBRATE” is displayed. To properly equilibrate the<br />
pumps, they must be delivering fluid.<br />
14. Once the message “Running” is displayed, modifier will be<br />
delivered at the selected rate.<br />
9.5 Refilling Once your modifier addition system is running, you will occasionally<br />
need to refill the pumps. The system must be stopped<br />
while the pumps are refilling. Pump A, the “CO 2 ” pump, may be<br />
refilled simply by designating the pump to be refilled and then<br />
pressing the REFILL key on the front panel (refer to instructions<br />
below). Pump B, “the modifier pump”, may also be refilled in this<br />
manner. However, if you wish to ensure that air is purged from<br />
pump B, it must be placed under independent control before<br />
refilling. Once under independent control, the pump will run<br />
until 5-10 ml of modifier are delivered back to the source (see<br />
refilling pump B below).<br />
To refill pump A (the CO 2<br />
pump)<br />
1. Press the STOP key.<br />
2. Close the outlet valves of both pumps.<br />
3. Press the REFILL key, and press softkey A to designate<br />
pump A.<br />
4. As soon as the pump pressure drops below the known tank<br />
pressure, open the tank supply valve and the pump A inlet<br />
valve.<br />
5. After pump A has refilled, the message “cylinder full” will<br />
be displayed on the controller front panel. Wait 15 seconds<br />
before closing the tank supply valve and the pump A inlet<br />
valve.<br />
6. Open the outlet valves of both pumps if they are not open.<br />
7. Press the RUN key on the controller front panel keypad.<br />
9-8
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