Invitro Cytotoxic Activity of Marsilea Quadrifolia Linn of MCF-7 ... - ISCA

International Research Journal of Medical Sciences ________________________________________________________________
Vol. 1(1), 10-13, February (2013)
Int. Res. J. Medical Sci.
Table-3
Invitro Cytotoxic Activity of Methanol, Ethylacetate and Aqueous Extracts of Marsilea quadrifolia linn
S.No Concentration
(µg/ml)
Dilutions Invitro cytotoxic activity on Breast cancer cell
lines (MCF 7 cell line)
5FU (Fluoro
uracil)
Methanol Aqueous Ethylacetate
1 1000 Neat 12.32 ±0.25 32.56 ±0.59 22.58 ±0.55 12.33 ± 0.51
2 500 1:1 15.96 ±0.89 41.23 ±0.32 29.63 ±1.25 17.18 ±1.58
3 250 1:2 18.96 ±1.23 48.92 ±0.57 35.47 ±1.35 20.98 ±1.47
4 125 1:4 25.36 ±0.89 52.35 ±0.44 40.21 ±1.47 24.13 ±0.55
5 62.5 1:8 38.59 ±1.05 59.87 ±0.14 48.96 ±0.25 28.78 ±0.87
6 31.25 1:16 52.55 ±1.47 61.23 ±0.57 51.22 ±0.45 37.17 ±0.69
7 15.625 1:32 58.79 ±1.69 68.77 ±0.22 54.69 ±1.25 43.46 ±0.54
8 10 1:64 65.32 ±0.58 72.02 ±1.06 61.23 ±1.88 48.19 ±1.25
9 7.8125 1:128 78.19 ±0.47 75.12 ±1.08 68.90 ±1.98 54.33 ±1.06
10 3.125 1:256 90.23 ±0.55 85.64 ±1.89 75.88 ±0.58 61.22 ±0.88
11 Cell control - 100 100 100 100
Table-4
Invitro Cytotoxic Activity Showing IC 50 values of MQ
S. No Extract IC 50 Concentration (µg/ml )
1. Methanol extract 39.06
2. Aqueous extract 187.5
3.
Ethyl acetate
extract
47.825
Conclusion
The results of this study revealed that methanolic extract of
M.quadrifolia contains pharmacologically active substances
with cytotoxic activity and it showed most effective inhibition
of MCF 7 cell proliferation. Accumulating evidence clearly
indicates that apoptosis is a critical molecular target by dietary
bioactive agents, in the prevention of cancer. Since the
phytochemical analysis has shown the presence of potent
phytochemicals like alkaloids, phenols, flavonoids,
terpenoids, glycosides, saponin, steroids, tannin and sugars
etc.Several authors reported that phenolic, acids, flavonoids,
steroids, terpenoids are known to be bioactive
principles 14,`15 . Therefore the crude extracts of M.quadrifolia
leaf could be new sources of development of new plant
based therapy for management of diseases. Further research
is to be carried out to fractionate and purify the extract, in
order to find out the molecules responsible for the antiproliferation
activity observed.
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