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C - Lublin

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N2O-N [mg kg -1 ]<br />

100<br />

80<br />

60<br />

40<br />

20<br />

0<br />

-20<br />

y = -12.073Ln(x) +<br />

23.933<br />

R 2 = 0.336***<br />

0 2 4 6 8 10<br />

O 2 [%]<br />

Total N 2O-N [mg kg -1 ]<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

170 190 210 230 250 270<br />

Eh [mV]<br />

Fig. 1. N 2 O emission vs. O 2 content<br />

for the day of maximum emission.<br />

Fig. 2. N 2 O emission as a function<br />

of Eh value (●emission ○absorption).<br />

Organic matter availability Denitrification is a respiratory process, which<br />

requires an easily oxidisable organic substrate. The presence of readily metabolize<br />

organic matter and the availability of water soluble organic matter are closely<br />

correlated with the rate biological denitrification and hence the potential production<br />

of N 2 O from soil. There is observed very high correlation between N 2 O emission<br />

and organic matter content (Fig. 3 Włodarczyk, 2000).<br />

Dehydrogenases These enzymes conduct a broad range of oxidative activities<br />

that are responsible for degradation, i.e., dehydrogenation, of organic matter. The<br />

amount of nitrous oxide formed due to denitrification showed high positive<br />

correlation with dehydrogenase activity (Fig. 4 Włodarczyk et al., 2001).<br />

N2O-N mg kg -1 d -1<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

y = 24.699x - 11.924<br />

R 2 = 0.946***<br />

N2O-N mg kg -1<br />

250<br />

200<br />

150<br />

100<br />

50<br />

y = 671.55x 0.84<br />

R 2 = 0.51**<br />

-10<br />

0 0,5 1 1,5 2 2,5<br />

0<br />

O.M %<br />

0 0,1 0,2 0,3<br />

nmol formazan g -1 min -1<br />

Fig.3 N 2 O emission as a function<br />

of organic matter content.<br />

Fig.4 N 2 O emission as a function<br />

of dehydrogenase activity.<br />

160

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