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Zooplankton of the open Baltic: Extended Atlas - IOW

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3. METHODS OF COLLECTING AND ANALYSING<br />

ZOOPLANKTON IN THE BALTIC SEA<br />

3.1. Sampling: general aspects<br />

Miscellaneous processes, like seasonality, daily vertical migration,<br />

swarming, etc. produce typical zooplankton distribution patterns (Haury et al.,<br />

1978). In reality, we observe a result <strong>of</strong> <strong>the</strong> combination <strong>of</strong> those processes,<br />

and skilful observation strategies need to be applied in order to distinguish<br />

<strong>the</strong>m by choosing proper sampling duration (length) and measuring intervals<br />

(Sameoto et al., 2000). Appropriate measuring intervals are to be chosen<br />

considering <strong>the</strong> so called Nyquist sampling <strong>the</strong>orem (Nyquist, 1928).<br />

Following it, a signal must be measured in equal distances <strong>of</strong> more than two<br />

times within one period (or wave length) <strong>of</strong> <strong>the</strong> specific signal. O<strong>the</strong>rwise one<br />

produces aliased results as Figure 3.1 illustrates.<br />

140<br />

Measurements <strong>of</strong> a 24 h periodicity by different<br />

sampling intervals<br />

120<br />

Abitrary units<br />

100<br />

80<br />

60<br />

40<br />

24 h periodicity<br />

8 h sampling interval<br />

13 h sampling interval<br />

28 h sampling interval<br />

20<br />

0<br />

0 24 48 72 96 120 144<br />

Time [h]<br />

Figure 3.1.1. Examples <strong>of</strong> sampling intervals producing accurate (by<br />

8 h) and biased (by 13 and 28 h) results <strong>of</strong> a 24 h periodicity.<br />

Nearly accurate results will be obtained if a certain process will be<br />

measured at least three times within its periodicity or a wave length. For<br />

example, <strong>the</strong> twenty four hour periodicity <strong>of</strong> dial vertical migration demands<br />

an equidistant measuring interval <strong>of</strong> at least eight hours (Fig. 3.1.1). Longer<br />

distances, like thirteen or twenty-eight hours, produce apparent results <strong>of</strong> a<br />

thirty-six hour or even a seven day periodicity, for example. Continuous<br />

measurements would produce <strong>the</strong> most accurate outcome in both phase and<br />

21

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