A homolog of the vaccinia virus D13L rifampicin resistance gene is ...
A homolog of the vaccinia virus D13L rifampicin resistance gene is ...
A homolog of the vaccinia virus D13L rifampicin resistance gene is ...
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Journal <strong>of</strong> Insect Science | www.insectscience.org ISSN: 1536-2442<br />
Figure 4b. ClustalW 1.81 multiple sequence alignment <strong>of</strong> <strong>the</strong> deduced amino acid sequence <strong>of</strong> a selected region <strong>of</strong><br />
<strong>the</strong> putative <strong>rifampicin</strong> <strong>res<strong>is</strong>tance</strong> protein <strong>homolog</strong>ues from entomopox<strong>virus</strong>es, showing regions I and II<br />
(underlined in HaEPV and DlEPV) <strong>of</strong> highest percent conserved sequences (Osborne et al. 1996) and <strong>the</strong>ir<br />
component motifs. Virus names, symbols, and color codes are as described in Fig. 4a.<br />
chordopox<strong>virus</strong>es and entomopox<strong>virus</strong>es but<br />
contained substitutions that d<strong>is</strong>tingu<strong>is</strong>hed <strong>the</strong> two<br />
<strong>virus</strong> subfamilies (Figure 4a). A closer analys<strong>is</strong> <strong>of</strong><br />
<strong>the</strong> entomopox<strong>virus</strong>es revealed that within <strong>the</strong><br />
motif in region I, DlEPV had a single substitution<br />
that d<strong>is</strong>tingu<strong>is</strong>hed it from <strong>the</strong><br />
betaentomopox<strong>virus</strong>es (Figure 4b). However, all<br />
residues in <strong>the</strong> motif in region II were conserved<br />
among all entomopox<strong>virus</strong>es (Figure 4b).<br />
Overall, pairw<strong>is</strong>e compar<strong>is</strong>on <strong>of</strong> amino acids <strong>of</strong><br />
DlEPV RIF with each <strong>homolog</strong> revealed that<br />
DlEPV shared slightly more amino acid identities<br />
with <strong>the</strong> betaentomopox<strong>virus</strong>es than with<br />
chordopox<strong>virus</strong>es (Table 1). However, <strong>the</strong><br />
betaentomopox<strong>virus</strong>es shared 1.5-2 times more<br />
amino acids among <strong>the</strong>mselves than <strong>the</strong>y did with<br />
DlEPV and <strong>the</strong> lepidopteran entomopox<strong>virus</strong>es<br />
shared more with each o<strong>the</strong>r than <strong>the</strong>y did with<br />
<strong>the</strong> M. sanguinipes entomopox<strong>virus</strong> (Table 1).<br />
The percent similarities between DlEPV and all<br />
pox<strong>virus</strong> RIF sequences and between <strong>the</strong><br />
betaentomopox<strong>virus</strong>es and chordopox<strong>virus</strong>es<br />
were about <strong>the</strong> same (on average ~44%) (Table 1).<br />
However, similarities among <strong>the</strong><br />
betaentomopox<strong>virus</strong>es were 1.5- 2 times higher<br />
than with DlEPV. The lepidopteran<br />
entomopox<strong>virus</strong>es had greater similarity with<br />
each o<strong>the</strong>r than with <strong>the</strong> M. sanguinipes<br />
entomopox<strong>virus</strong> (Table 1).<br />
The nucleotides conserved between DlEPV and<br />
<strong>the</strong> betaentomopox<strong>virus</strong>es were 1.5 to > 5x fewer<br />
than those conserved among <strong>the</strong><br />
betaentomopox<strong>virus</strong>es <strong>the</strong>mselves, with <strong>the</strong><br />
lepidopteran entomopox<strong>virus</strong>es sharing more<br />
with each o<strong>the</strong>r than with <strong>the</strong> M. sanguinipes<br />
entomopox<strong>virus</strong> (Table 1). Never<strong>the</strong>less, both<br />
DlEPV and <strong>the</strong> betaentomopox<strong>virus</strong>es had few (0-<br />
≤ 20%) nucleotide identities with <strong>the</strong><br />
chordopox<strong>virus</strong>es, except in <strong>the</strong> case <strong>of</strong> <strong>the</strong> A.<br />
moorei entomopox<strong>virus</strong> and swinepox (Table 1).<br />
Thus, <strong>the</strong> DlEPV putative RIF protein <strong>is</strong> closer to<br />
(but d<strong>is</strong>tinct from) <strong>homolog</strong>s <strong>of</strong> <strong>the</strong> lepidopteran<br />
and orthopteran entomopox<strong>virus</strong>es than to those<br />
<strong>of</strong> chordopox<strong>virus</strong>es (Table 1). Th<strong>is</strong> <strong>is</strong> fur<strong>the</strong>r seen<br />
in <strong>the</strong> phylo<strong>gene</strong>tic tree that assigns DlEPV to a<br />
different clade from <strong>the</strong> M. sanguinipes<br />
entomopox<strong>virus</strong> and from <strong>the</strong> H. armigera and A.<br />
moorei entomopox<strong>virus</strong>es (Figure 5). DlEPV had<br />
~20% and 26.4% similarity respectively, with<br />
IIV-6 and DpAV4a, two non-pox double stranded<br />
DNA <strong>virus</strong>es <strong>of</strong> insects ≤22.% with non-pox<br />
double stranded DNA <strong>virus</strong>es <strong>of</strong> o<strong>the</strong>r organ<strong>is</strong>ms<br />
(Table 2).<br />
Journal <strong>of</strong> Insect Science: Vol. 8 | Article 8 11