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Molecular Systematics of Nematodes - Russian Journal of Nematology

Molecular Systematics of Nematodes - Russian Journal of Nematology

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Blast (Sequence Similarity Search)<br />

1. Point your browser to the NCBI BLAST<br />

server<br />

at:<br />

http://www.ncbi.nlm.nih.gov/BLAST<br />

2. Under the Nucleotide heading, click the<br />

Nucleotide-Nucleotide (blastn) link<br />

3. Paste your sequence in the search<br />

window<br />

4. Click Blast! button<br />

5. Click Format button (and wait)<br />

An overview <strong>of</strong> the BLAST output<br />

1. A graphic display: Shows you where<br />

your query is similar to other sequences<br />

2. A hit list: The name <strong>of</strong> sequences<br />

similar to your query, ranked by<br />

similarity<br />

3. The alignments: Every alignment<br />

between your query and the reported<br />

hits<br />

4. The parameters: A list <strong>of</strong> the varios<br />

parameters used for the search

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