Molecular Systematics of Nematodes - Russian Journal of Nematology

More documents

Recommendations

Info

Blast (Sequence Similarity Search) Sequence alignments provide a powerful way to compare novel sequences with previously characterized genes. Both functional and evolutionary information can be inferred from well designed queries and alignments. BLAST 2.0, (Basic Local Alignment Search Tool), provides a method for rapid searching of nucleotide and protein databases. BLAST - the most popular datamining tool ever! For non-coding DNA - use blastn. Never forget that blastn is only for closely related DNA sequences (more than 70 percent identical).
Blast (Sequence Similarity Search) 1. Point your browser to the NCBI BLAST server at: http://www.ncbi.nlm.nih.gov/BLAST 2. Under the Nucleotide heading, click the Nucleotide-Nucleotide (blastn) link 3. Paste your sequence in the search window 4. Click Blast! button 5. Click Format button (and wait) An overview of the BLAST output 1. A graphic display: Shows you where your query is similar to other sequences 2. A hit list: The name of sequences similar to your query, ranked by similarity 3. The alignments: Every alignment between your query and the reported hits 4. The parameters: A list of the varios parameters used for the search
Page 1 and 2: Molecular Systematics of Nematodes
Page 3 and 4: GenBank
Page 5 and 6: GenBank from 1982 to the present, t
Page 7 and 8: GenBank
Page 9 and 10: GenBank PubMed: Allows searching by
Page 11 and 12: GenBank Nucleotide Database: The nu
Page 13 and 14: GenBank Taxonomy Browser is… brow
Page 15: Blast (Sequence Similarity Search)
Page 19 and 20: GenBank 1. Locus gives us the locus
Page 21 and 22: TreeBase TreeBASE is a relational d
Page 23: Useful databases for nematologists

Molecular Systematics of Nematodes - Russian Journal of Nematology

Create successful ePaper yourself

Delete template?

Save as template?