L. Lawsonia inermis

125 - 113
:
1
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Lawsonia inermis L.
(1)
Fusarium oxysporum f.sp. melonis
300 )
(
% 80
mauginiella scaettae
300)
M. Scaettae
(PD Broth)
Rhizoctonia solani
T. paradoxa
(PDA)
F. Oxysporum
(
Lawsonia inermis
thielaviopsis paradoxa
140)
(
(
R. solani
)
.%83
.
:
.
(1)
113

Lawsonia inermis L.
Study antifungal activity of Henna leaves
(Lawsonia inermis) extracts on some plant
pathogenic fungi
Mohammed Hamza Abass (1)
ABSTRACT
This study was conducted to determine the effect of different
concenterations of Henna (Lawsonia inermis) leaves extracts on some plant
pathogenic fungi which were Fusarium oxysporum f.sp. melonis; Thielaviopsis
paradoxa; Rhizoctonia solani and Mauginiella scaettae. Results showed the high
antifungal activity of Henna leaves extracts, this was evident with high
concenteration of extract (300 ppm), which completely inhibited the radial
growth on both solid and liquid media (PDA and PD Broth) of the pathogens F.
oxysporum f.sp. melonis; T. paradoxa and R. solani, also the fungal spores of F.
oxysporum f.sp. melonis; T. paradoxa failed to germinate in the concenteration
300 ppm of the extract. The results proved that the minimum inhibitory
concenteartion of extract against the radial growth of R. solani was 140 ppm,
the extract had no toxic effect on the cabbage seeds germination when treated
with 5 ml of crude extract, the germination percentage was 80%,while it was
83% in control treatment.
Key words: Antifungal activity, Lawsonia inermis, Plant pathogenic
fungi.
(1) Date Palm Research Center-Basrah Univ, Iraq.
114

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:
1
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Lythraceae
Lawsonia inermis
.
4--2
.(1989
1981
)
(1985 )
Antimicrobial activity
Helminthosporium sp.
Fusarium sp. Ustilago trilago Curvularia lunata Dreschlera rostrata
Wangkiat,1995;Misra&Dixit,1979; Hamadi, ) Pythium sp. Colletotrichum sp.
Xanthomonas campestris
(1978
Korayem and ) Meliodogyne incognita
(Satish et al.,1999)
(Osman,1992
Zea mays
(1986 )
Aspergillus fumigatus
Al-Mayah and Al-wailly, 2002)) %80
Lyopersicon esculentum ( )
(Tripathi et al., 1978)
.(Suberu, 2004)
/ -
.
115

Lawsonia inermis L.
:
Fusarium oxysporum f.sp. melonis
Vascular wilt (Cucumis melo)
Thielaviopsis paradoxa
-
(Phoenix dactylifera)
Terminal bud rot
Rhizoctonia solani
-
Damping-off (
)
Mauginiella scaettae
.Inforescenc rot
:
- 1
- 2
2004
.
Magnetic stirrer
500
:
- 3
100
(%99.99)
(
:
°37
Whatman No.1
Buchi (Switzerland) Rotary evaporator
°45
2004
.(2003)
Antifungal activity
300
200
100
:
Stock solution
0)
- 4
- 1- 4
116

(2007)
(23)
1
:
113
-
125
117
Potato Dextrose Agar(PDA)
(2004)
.
:
)
(
-
%
=
x
100
4
2-
-
:
PD Broth
0.5
°30
10
°85
(Suberu,2004)
4
.1-
4
3-
-
:
4
1-
05.
10
4.5
:
:
:
(F.A.A)
:1:1
8
Haemocytometer
(2004)
0.5
(PDA)
4.5
9
10
2-
Water agar (2%)
°30
.
48
25
.

Lawsonia inermis L.
- 5- 4
:R.solani
.
(
)
1- 4
%10
9
( Iqbal et al., 2004)
10
5
:
- 5
100
200
PDA
.
- 6- 4
:(Brassica oleraceae)
10
25
5
°25
.(1998 )
L.S.d
.(1980
6 5
Arcsine transformation
) % 1
- 1
(%35.55)
(1)
R.solani
.M. scattae
%87.22
T .paradoxa
118

125 - 113
:
1
(23)
(2007)
(
)
(1)
(ppm)
300 200 100
57.10 100.00 53.32 18.00 F.oxysporum f.sp.melonis
70.10 100.00 73.00 37.32 T.paradoxa
87.22 100.00 100.00 61.67 R.solani
35.55 61.67 33.32 11.67 M.scaettae
90.40 64.90 32.16
2.4
=
3.5 =
4.1 =
L.S.D. (0.01)
100
F.oxysporum f.sp. melonis
300
F.oxysporum f.sp. melonis
%11.67 18
(
%32.16
300 % 90.40
R. solani
300 200 )
PDA
T. paradoxa
100 M. scattae
.
300
200
100
Lawsone
)
2-hydroxy, 1-4-naphthoquinone
.(2003
helminthosporium sp. curvularia pennisti Altrernaria tenuis :
(Shekhawat and Prasada, 1971) %100
fusarium sp. Colletotrichum sp.
%50
.(Wangkiat, 1995) pythium sp.
119

Lawsonia inermis L.
- 2
(2
)
PD Broth
.(%46.10)
M. scattae
300
R. solani
200 100
(
)
PD
(ppm)
300 200 100
73.10 100.00 76.67 42.67 F.oxysporum f.sp.melonis
76.65 100.00 88.32 41.67 T. paradoxa
100.00 100.00 100.00 100.00 R. solani
46.10 70.00 50.00 18.32 M. scaettae
92.50 78.73 50.67
.2 =
3.5 =
4.1=
L.S.D. (0.01)
(2)
100
.
M. scattae
Iqbal et al. (2004)
)
(Dixit et al.,1978) Nitrate reductase
Phomopsis thea Fusarium sp. R. solani
150 Ageratum conyzoides
.(2003
- 3
120

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:
1
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(2007)
F. oxysporum f.sp.
(2 1)
300
(%80.32
.
.
T. paradoxa melonis
72.43)
T. paradoxa
M scaettae
F.o.m.
T.paradoxa
M.scattae
4
300
3
200
2
100
1
100
90
80
70
60
50
40
30
20
10
0
(1)
Aspergillus fumigatus Microdochium nivale Sphaerotheca fuliginea
Mohamoud Jadhave et al., 1997 Kurucheve et al. , 1997) Botrytis fabae
(et al. ,2004,Al-Mayah and Al-waily,2002 ,Coventry and Allan, 2001
121

Lawsonia inermis L.
.(Dubey and Dwivendi,1991)
100
90
80
70
60
50
40
30
20
10
S1 0
S2 S3
2 1
S4
4 3
5
300
200
R. solani 100
( 3)
%90
140
(Iqbal et al., 2004)
M .scaettae
130
T.parada
F.om
R.solani
140 200
(2)
- 4
.(Ghannoum and Rie, 1999)
122

(2007)
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1
:
113
-
125
123
100
110
120
130
140
150
160
170
180
190
200
0
10
20
30
40
50
60
70
80
90
100
(3)
R. solani
5
-
:
)
(
%80
5
%83
)
(1998
Tripathi et al.(1978)
400
.
.
R.L.S.D.( 0.01)=2.5

Lawsonia inermis L.
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.
.
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Received 2005 /08/ 30
Accepted for Publ. 2006 /04/ 04
125