MATERIAL SAFETY DATA SHEET - Illumina

TruSeq Enrichment Kit Illumina Doc. #15018476, Rev. A Page 10 of 17 

12. ECOLOGICAL INFORMATION 

ALL WORK PRACTICES MUST BE AIMED AT ELIMINATING ENVIRONMENTAL CONTAMINATION. 

MOBILITY: this product has not been tested for mobility in soil. The following information is available for some 

constituents. 

ALIPHATIC AMIDE: 

The Koc of Aliphatic Amide is 3.6. According to a classification scheme, this Koc value suggests that Aliphatic Amide is expected to have very high mobility in soil. 

ALKALI HYDROXIDE: 

The Octanol/Water Partition Coefficient of Alkali Hydroxide is too low to be measured (or possibly virtually 0). In aqueous solutions, Alkali Hydroxide will be highly 

mobile. 

PERSISTENCE AND BIODEGRADABILITY: This product has not been tested for persistence or biodegradability. It 

is expected that the constituents of this product will slowly degrade in the environment and form a variety of organic 

and inorganic materials; however, no specific information is known. Data for some constituents of this product are 

available as follows: 


If released to air, a vapor pressure of 6.1X10-2 mm Hg at 25°C indicates Aliphatic Amide will exist solely as a vapor in the ambient atmosphere. Vapor-phase 

Aliphatic Amide will be degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals; the half-life for this reaction in air is estimated 

to be 8.0 days. If released to soil, Aliphatic Amide is expected to have very high mobility based upon a Koc of 3.6. Volatilization from moist soil surfaces is not 

expected to be an important fate process based upon an estimated Henry's Law constant of 1.4X10-9 atm-cu m/mole. If released into water, Aliphatic Amide is 

not expected to adsorb to suspended solids and sediment based upon the Koc. Several biodegradation screening studies have observed significant 

biodegradation of Aliphatic Amide which suggests that biodegradation may be important. Volatilization from water surfaces is not expected to be an important 

fate process based upon this compound's estimated Henry's Law constant. Hydrolysis is expected to be slow. 

SODIUM SALT: 

Water solubility = 37 g/ 100 mL @ 0°C; 39.12 g/100 ml of water @ 100°C; Log Kow = -3.0 


Persistence and Biodegradability: In the case of a solid, anhydrous Alkali Hydroxide spill on soil, ground water pollution will occur if precipitation occurs prior to 

clean up. Precipitation will dissolve some of the solid (with much heat given off) and create an aqueous solution of Alkali Hydroxide, which then would be able to 

infiltrate the soil. However, prediction of the concentration and properties of the solution produced would be difficult. No biodegradation of Alkali Hydroxide 

occurs. 

BIO-ACCUMULATION POTENTIAL: This product has not been tested for bio-accumulation potential. The following 

information is available for some constituents. 


An estimated BCF of 3 was calculated for Aliphatic Amide, using a log Kow of -1.51 and a regression-derived equation. According to a classification scheme, this 

BCF suggests the potential for bioconcentration in aquatic organisms is low. 


Bioconcentration is unlikely. 

ECOTOXICITY: This product has not been tested for aquatic or animal toxicity. All releases to terrestrial, 

atmospheric and aquatic environments should be avoided. The aquatic toxicity data for some constituents of this 

product are available on the following below. 


LC50 (minnow) > 500 mg/L/ 48 hours 

CHLORIDE SALT: 

EC50 (Daphnia magna Water flea) 24 hours = 15.12 

mM; Conditions: freshwater; static; Effect: 

intoxication, immobile 

LC50 (Ceriodaphnia dubia Water flea) 48 hours = 

630,000 µg/L (95% confidence limit: 580,000 to 

670,000 µg/L), Conditions: freshwater; static 

LC50 (Ceriodaphnia dubia Water flea) 48 hours = 


630,000 µg/L), Conditions: freshwater; static 

LC50 (Chironomus riparius Midge); 96 hours = 4.81 g/L 

(95% confidence limit: 3.93 to 5.68 g/L), Conditions: 

freshwater; /conditions of bioassay not specified 

LC50 (Chironomus tentans Midge, size 1.56 mm, 1st 

instar); 96 hours = 1,250,000 µg/L, Conditions: 

freshwater; static 

LC50 (Chironomus tentans Midge, size 4.42 mm, 2nd- 

3rd instar) 96 hours = 1,770,000 µg/L (95% 

confidence limit: 590000 to 5,260,000 1,250,000 

µg/L g/L); Conditions: freshwater; static 

LC50 (Chironomus tentans Midge, size 6.07 mm, 3rd 

instar) 96 hours = 2,890,000 µg/L (95% confidence 

limit: 2,390,000 to 3,500,000 µg/L); Conditions: 














LC50 (Chironomus tentans Midge, size 9.41 mm) 96 

hours = 5,300,000 µg/L (95% confidence limit: 

4,330,000 to 6,520,000 µg/L); Conditions: 


LC50 (Chironomus tentans Midge, size 8.67 mm) 96 

hours = 5,360,000 µg/L (95% confidence limit: 

4,430,000 to 6,490,000 µg/L); Conditions: 


CHLORIDE SALT (continued): 

LC50 (Chironomus tentans Midge, size 10.87 mm, 3rd- 

4th instar) 96 hours = 6,190,000 µg/L (95% 

confidence limit: 5,370,000 to 7,130,000 µg/L); 

Conditions: freshwater; static 

LC50 (Chironomus tentans Midge, size 9.42 mm, 3rd- 

4th instar) 96 hours = 6,200,000 µg/L (95% 











LC50 (Daphnia magna Water flea) 96 hours = 29 mg/L; 


LC50 (Daphnia magna Water flea) 72 hours = 117 

mg/L; Conditions: freshwater; static 

EC50 (Daphnia magna Water flea) 24 hours = 7350 

µmol/L; Conditions: freshwater; /conditions of 

bioassay not specified/; Effect: intoxication, 

immobile 

EC50 (Daphnia magna Water flea) 48 hours = 141,460 

µg/L (95% confidence limit: 95300 to 170700 µg/L); 

Conditions: freshwater; static, Effect: intoxication, 

immobile 

EC50 (Daphnia magna Water flea 24 hours = 327,940 

µg/L (95% confidence limit: 248,600 to 407,200 

µg/L); Conditions: freshwater; static; Effect: 

intoxication, immobile 

LC50 (Daphnia magna Water flea, 4th instar or adult) 

24 hours = 343,000 µg/L; Conditions: freshwater; 

static 

LC50 (Daphnia magna Water flea, 4th instar or adult) 


static; Concentration 

LC50 (Daphnia magna Water flea, < 24 hr) 48 hours = 


880,000 µg/L); Conditions: freshwater; static 

LC50 (Daphnia magna Water flea, < 24) 24 hours = 


880,000 µg/L); Conditions: freshwater; static 

CHLORIDE SALT (continued): 

LC50 (Hyalella azteca Scud) 96 hours = 0.41 g/L (95% 

confidence limit: 0.35 to 0.49 g/L); Conditions: 

freshwater; flow-through 

LC50 (Hyalella azteca Scud) 48 hours = 0.54 g/L; 

Conditions: freshwater; flow-through 

LC50 (Hyalella azteca Scud, size 1.85 mm) 96 hours = 

0.54 g/L (95% confidence limit: 0.47 to 0.61 g/L); 


LC50 (Hyalella azteca Scud) 72 hours = 0.63 g/L; 


LC50 (Hyalella azteca Scud) 96 hours = 134,000 µg/L; 

Conditions: freshwater; renewal; formulated product 

LC50 (Hyalella azteca Scud) 96 hours = 141,900 µg/L 

(95% confidence limit: 100,700 to 199,800 µg/L); 


LC50 (Gambusia affinis Western mosquitofish, female) 


static 


48 hours = 1,990,000 µg/L; Conditions: freshwater; 

static 


24 hours = 4,700,000 µg/L; Conditions: freshwater; 

static 

LC50 (Lepomis macrochirus Bluegill, size 5.3-7.2 cm, 

wt 3.5-3.9 g) 96 hours = 2,010,000 µg/L; Conditions: 

freshwater; static; Effect: mortality, survival 

LC50 (Oncorhynchus mykiss Rainbow trout, donaldson 

trout, size 5.0-6.0 cm) 24 hours = 1191000 µg/L 

(95% confidence limit: 923,000 to 1,536,000 µg/L); 


LC50 (Oncorhynchus mykiss Rainbow trout, donaldson 

trout, wt 0.8-1.2 g) 48 hours = 1,610,000 µg/L (95% 



LC50 (Pimephales promelas Fathead minnow) 96 hours 

= 880,000 µg/L (95% confidence limit: 750,000 to 

1,020,000 µg/L) 880,000 µg/L (95% confidence limit: 

750,000 to 1,020,000 µg/L); Conditions: freshwater; 

static 

LC50 (Pimephales promelas Fathead minnow) 48 hours 

= 910,000 µg/L (95% confidence limit: 750,000 to 

1,090,000 µg/L); Conditions: freshwater; static

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MATERIAL SAFETY DATA SHEET - Illumina

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