INDIAN SCIENCE CONGRESS - India Environment Portal

More documents

Recommendations

Info

Section XIV : Plant Sciences 163 In vitro mass propagation has been a well established technique that is capable of producing large scale quality planting material with reproducibility and efficiency. However, owing to intensive use of skilled manpower, electricity, high grade chemicals and reagents etc. , the tissue culture technology proves expensive. And many a times, high per plant cost of tissue cultured plants reduces its acceptability. Of the media components, the gelling agents such as agar contribute 70% of the cost. Therefore, agar is the major component to be replaced to reduce. Several attempts have been made to reduce the cost of tissue culture media components using low cost alternatives to agar (gelling agent) and sucrose (carbon source). Isabgol-husk, guar gum and sago powder were used as low cost gelling alternatives and table sugar, raw sugar and jaggary were tested as low cost carbon sources in tissue culture media. We have tested these low cost alternatives during in vitro rooting of microshoots of two plants of arid and semi-arid regions of India -Balanites aegyptiaca and Phyllanthus emblica. Gelling agent alternatives used were- sago powder, guar gum and isabgol husk that solidify media at 15. 0% (w/ v), 5. 0% (w/v) and 5. 0% (w/v) respectively. In presence of isabgol, rooting response in micro shoots of Balanites aegyptiaca was found to be equivalent to that of agar and application of isabgol husk can reduce the cost of gelling agents per unit media by over 40%. Moreover, it has been found that table sugar can be used for in vitro rooting of both the species at commercial scale. As far as gelling agents is concerned isabgol was found to be a suitable gelling agent for rooting with equally good rooting response as that of agar. 205. Seed Germination and Berberine Content in Berberis aristata DC., growing in Himachal Pradesh, India Rajasekaran, Nilay Kumar and Joginder Singh Himalayan Forest Research Institute, Shimla, Himachal Pradesh-171 009 Keywords : Berberine, seed germination, Berberis aristata, HPLC Berberis aristata DC. (Family : Berberidaceae; Hindi : Daruharida, Rasaut; English : Indian Barberry) is one of the high medicinal value species of temperate areas. It is mostly found growing wild in the sub-Himalayan tract at altitude
164 Proc. 98th Indian Science Congress, Part II : Abstracts of Oral/Poster Presentation ranging from 2,150 to 2,800 meters. Rasaut (also called rasaunt or rasanjana) is a useful concentrated extract obtained from roots of this plant and is used in curing many human ailments. Moreover, the plant has hepatoprotective, antiturnour, sedative and wound healing properties. The chief constituent of the roots and stem bark of B. aristata is an alkaloid Berberine, which is reported to be I responsible for most of the biological activity of the plant. A study was conducted to identify high berberine yielding plants/populations and to assess the seed germination potential of the plant growing in Himachal Pradesh. Berberine content was estimated using High Performance Liquid Chromatography (HPLC). The chemical analysis showed maximum berberine content of 2. 81 per cent in sample No. 30 collected from Shimla district. Effect of different treatments on seed germination of the plant was also studied under laboratory conditions. The seed germination studies revealed that maximum germination (93. 50%) was recorded in seeds treated with hot water fot 24 hours. In this paper variation in berberine content and seed germination potential of B. aristata growing in Himachal Pradesh have been discussed. 206. A Comparison of Bacterial Cell Wall Disruption Techniques for the Xtraction of Intracellular Enzyme Wamik Azmi* and Sarita Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh-171005 Keywords : L-asparaginase, Erwinia carotovora MTCC1428, cell disruption, sonication. Disintegration of microbial cells is a necessary first step for the purification or extraction of intracellular enzymes and organelles. With increasing use of intracellular microbial materials in industry and medicine, the cell disruption unit operation is gaining in importance. L-asparaginase (E. C. 3. 5. 1. 1.) is a therapeutic enzyme and extensively used in the treatment of acute lymphoblastic leukemia. L-asparaginase is widely distributed among microorganisms, plants and animals. Due to the difficult extraction procedure to obtain the enzyme from plants and animals,
Page 1 and 2:
A-PDF Merger DEMO : Purchase from w
Page 3 and 4:
Page 5 and 6:
Section XIV : Plant Sciences 3 Plas
Page 7 and 8:
Section XIV : Plant Sciences 5 Sout
Page 9 and 10:
Section XIV : Plant Sciences 7 of m
Page 11 and 12:
Section XIV : Plant Sciences 9 acut
Page 13 and 14:
Section XIV : Plant Sciences 11 Onl
Page 15 and 16:
Section XIV : Plant Sciences 13 by
Page 17 and 18:
Section XIV : Plant Sciences 15 dif
Page 19 and 20:
Section XIV : Plant Sciences 17 rec
Page 21 and 22:
Section XIV : Plant Sciences 19 Cul
Page 23 and 24:
Section XIV : Plant Sciences 21 iii
Page 25 and 26:
Section XIV : Plant Sciences 23 in
Page 27 and 28:
Section XIV : Plant Sciences 25 com
Page 29 and 30:
Section XIV : Plant Sciences 27 8.
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Section XIV : Plant Sciences 33 103
Page 37 and 38:
Section XIV : Plant Sciences 35 133
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
2 Proc. 98th Indian Science Congres
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
10 Proc. 98th Indian Science Congre
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
98 th Indian Science Congress Janua
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
100 Proc. 98th Indian Science Congr
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208: 112 Proc. 98th Indian Science Congr
Page 257: 162 Proc. 98th Indian Science Congr
Page 271 and 272: A-PDF Merger DEMO : Purchase from w
show all

INDIAN SCIENCE CONGRESS - India Environment Portal

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?