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Section XIV : Plant Sciences 161 mutants were. Screened in M3inl contrast to the norma l looking fruit plants. A number. of yield component parameters were recorded including plant height, basal diameter, plant spread, root. length, pod per plant, seeds per pod, pod length /breadth ratio, number of primary branches number of secondary branches. leaf angle branching angle, first flowering date 100. flowering date to salduiatiop. ”pe~6entage of pollen sterility an4. weight of 100 seeds which Were found to vary from the conttol plant. Chromosome alaysis revea1ed aberrations like stickiness fragmentation polyplpidy clumping, laggard and bridge formation etc. 202. Plant Regeneration from Shoot Tip explants of Citrullus colocynthis (Linn.) Schrad. –An Important Medicinal Herb Mahesh Chand Meena, Ramraj Meena and U. Kant Plant pathology, Tissue culture and Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004 Keywords : Citrullus colocynthis, Medicinal Plant, Regeneration, Shoot tip A high frequency and rapid regeneration protocol was developed from shoot tip explants of Citrullus colocynthis on MS medium supplemented with BAP (0. 5 mg/l) and NAA (0. 5 mg/l). Highest number of shoots (23. 0 ± 0. 567) was obtained on MS medium containing BAP (0. 5 mg/l) and NAA (0. 5 mg/ l). The regenerated shoots were further elongated on same medium. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing IBA (4. 0 mg/l) with 0. 2% activated charcoal. The rooted plants were hardened in polycups containing sterile soil and vermiculate and finally well established in the field; survival rate was 60%. This is the first report of direct in vitro plantlet regeneration in Citrullus colocynthis from shoot tip explant.
162 Proc. 98th <strong>India</strong>n Science Congress, Part II : Abstracts of Oral/Poster Presentation 203. Sewage Water Irrigation : Impacts on Physical and Biochemical Parameters of Wheat (Triticum aestivum L.). Chandra Shekhar Kumar, R. K. Sagar and J. N Maurya Cell and Molecular Biology Lab, Department of Plant Science, M. J. P. Rohilkhand University, Bareilly-243006, U.P. Keywords : Sewage water, Wheat, Biochemical parameters A field experiment was conducted to study the physical and biochemical parameters of winter wheat (Triticum aestivum L.) variety PBW-343 grown under sewage water collected from Bareilly city (U. P) <strong>India</strong>. The effect of sewage water was analysed by measuring various physiochemical parameters. Crop irrigated with 100% sewage water showed increased number of tillers, leaf area per plant, no. of ears and seed yield per plant as compared to control. 50% sewage water irrigation also increased the growth of crop compared to tap water (control) irrigation, but increase was less than 100% sewage water. Various biochemical parameters such as Chlorophyll, Carotenoid, Protein and Proline were analysed. There was a significant increase in Chlorophyll and Protein content in wheat plant irrigated with 100% sewage water as compared to 50% sewage water and control. Carotenoid and Proline enhanced to a greater extent when wheat was irrigated with 100% sewage water as compared to control. The study reflects that sewage water can be reused for irrigation purpose in agriculture. 204. Economizing Tissue Culture Technology through Standardized Low Cost Media Component Alternatives Tarun Kant, Ashok Parmar and Vinod Singh Gour Biotechnology Laboratory, FGTB Division, Arid Forest Research Institute, New Pali Road, Jodhpur Keywords : microshoots, Balanites aegyptiaca
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