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Section XIV : Plant Sciences 159
199. Variation for Salt Tolerance Attributes In Csrio (Salt Tolerant, Indica)
X Taraori Basmati Derived Recombinant Inbred Lines
Vinita Bhankar l , Sunita Jain 2 *and Rajinder Kumar Jain 1
1 Oepartment of Biochemistry,
CCS Haryana Agricultural University,
Hisar-125004
2 Bioinfonnatics Section,
CCS Haryana Agricultural University,
Hisar-125004
Department of Biotechnology and Molecular Biology,
CCS Haryana Agricultural University,
Hisar-125004
Keywords : Basmati, linkage mapping, recombinant inbred lines, rice, salt tolerance.
A population of 208 recombinant inbred lines (RILs) derived from a cross
between CSRI0 (salt tolerant, indica) and Taraori Basmati (HBCI9) was
evaluated for various salt-tolerance attributes in a hydroponic culture system
using Yoshida nutrient solution containing 0 and 50 mM NaCI. Large variation
was observed in CSRI0 x Taraori Basmati RILs for shoot length (18. 4-45. 1
cm), root length (5. 8-34. 5 cm), fresh weight (0. 319-1. 276 g), dry weight (0.
083-0. 288 g), Na+/IC ratio (0. 033-0. 472), relative water content (RWC, 56. 1-
91. 6%) and osmotic potential (-0. 80 to -5. 72) in 35-day-old seedlings. The data
was used to calculate salinity tolerant indices and mean salinity score (on 1-9
scale; lower scale state for salt tolerance). The overall mean salinity score of the
CSRIO x HBC19 RILs ranged from 1. 5 to 8. 5 with a mean value of 4. 349
:1 :0. 089. Data on salt stress injuries including shoot tip burning, yellowing of
leaves and leaf curling showed CSRI0 as highly tolerant (score 1. 5) and HBC19
as highly sensitive (score 8. S); the score of RILs ranged between 1 and 9 with
a mean value of 4. 211 :i :0. 110. Three of the RILs showed transgressive
segregation for salt tolerance. The results Showed large variability for various salt
tolerance attributes in the RIL population and hence it can be used further for
linkage mapping and QTL analysis.