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Section XIV : Plant Sciences 155 eration, and elongation. Rooted shootlets were transferred for hardening in plastic pots containing the mixture of sand, soil and vermin compost in the ratio of 1 :1 :1 provided with Knops solution. These pots were placed in the plant growth chamber with temperature of 23 0 C and Humidity 85%. Callogenesis was observed with 2, 4-D, KN, NAA, BAP and GA 3 in combinations. NAA 0. 05 mg L -1 and BAP 0. 1 mg L -1 ; NAA 0. 05 mg L -1 + BAP 0. 1 mg L -1 + Kn; NAA 0. 05 mg L -1 + BAP 1. 0 mg L -1 ; 2, 4- D 1 mg L -1 showed significant callus initiation and proliferation than other concentrations used. The results revealed that BAP was ideal for good establishment individually and also in combination with NAA and KN. BAP in combination with NAA was found effective for multiplication. Individual treatment of BAP elicited good elongation. IBA was found to induce the initiation and proliferation of roots. 195. Phytosterol Production in Suspension Cultures of Acacia nilotica (Linn.) Padmasri Govindarajan and D. V. L. Sarada Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai-603203, Tamil Nadu Keywords : Acacia nilotica, Mimosaceae, Phytosterol, Gamborg’s medium The present study describes the effect of light conditions and pH on the production of phytosterols in suspension cultures of Acacia nilotica. Callus cultures were initiated from leaf explants of Acacia nilotica on B5 (Gamborg) medium amended with four different concentrations of 2,4-D, NAA and IBA namely, 0. 25, 0. 5, 1. 0 and 2. 0 mg/L in combination with Kinetin and BA at 0. 5mg/L at 16 :8 photoperiod and 25ºC. Profuse callus was initiated at NAA (2. 0mg/L) and BA (0. 5mg/L). The callus was transferred to B5 liquid medium amended with NAA (2. 0mg/L) and BA (0. 5mg/L). Experiments were carried out under continuous light, in dark and at different pH, namely 3. 7, 4. 2, 5. 6, 6. 5. The effect of pH was studied at 16 : 8 light dark cycle. Biomass and Phytosterol production in the suspension cultures were quantified upto a period of 21 days at regular intervals. Production of phytosterols was high in dark grown cultures when compared to
156 Proc. 98th <strong>India</strong>n Science Congress, Part II : Abstracts of Oral/Poster Presentation cultures grown in continuous light. Maximum phytosterol production was observed at pH 5. 6. In the light and dark grown cultures there existed a direct correlation between biomass and phytosterol production which was not the case in cultures grown at different pH. 196. Establishment of an in vitro Grafting system for Rose Shubham Chakravarty 1 , Sankarshan Sen 1 and Siraj Datta AJC Bose Laboratory for Plant Biotechnology, School of Biotechnology and Life Sciences, Haldia Institute of Technology, Haldia, West Bengal-721657 Keywords : Micro propagation, Micro grafting, Rosa hybrida, Coir The major limitation in the cultivation of Rose is that, the elite cultivars are of significantly less vigor when grown on their own roots. On the other hand, when Rose is propagated by the traditional ex vitro budding or grafting techniques, disease-free shoot and root stocks, suitable for grafting, are not sufficiently available. Therefore, the key to propagate a large number of Rose plants of sufficient viability relies on the establishment of a suitable in vitro grafting system for Rose. In order to develop an efficient micro grafting protocol for Rose, various aspects of in vitro grafting in Rose were studied using four different varieties of Rose. First, the four types of Rose, which included three elite cultivars, Rosa hybrida cv Sanjith, Rosa hybrida cv Revolution X Bicolette, Rosa hybrida cv East-West and a suitable root stock, Rosa multiflora, were micro propagated to produce disease-free plants, suitable for grafting, in large numbers. Then each of the three elite cultivars were grafted in vitro onto the root stock and the parameters like shoot-root combination, scion and root stock age and length were studied to increase the efficiency of micro grafting. The survival of grafts was higher when Rosa hybrida cv Sanjith and Rosa hybrida cv East-West were grafted onto Rosa multiflora than when Rosa hybrida cv Revolution X Bicolette was grafted onto the same root stock. Scion size size of 0. 6–1. 5 cm
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