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Section XIV : Plant Sciences 133 Cochlospermum religiosum (L.) is extensively used in Ayurvedic medicines and other uses. Every part of this plant is used medicinally. According to the survey of (UNDP) (CAMP), 2007, 39 medicinal plants of Rajasthan have been Red listed. Out of these, Cochlospermum religiosum is one of the critically endangered medicinal plant. This plant is thermogenic, sedative and used in cough, diarrhea, dysentery, pharyngitis, gonorrhea, syphilis and trachoma. It is also used for its anti inflammatory activity in Siddha Drug ‘Kalnar Parpam’. Gum katira is used as a cheap gelling agent for plant tissue culture and has wide application in pharmaceutical and food industries. During the present investigation shoot tip and nodal segment were used for tissue culture studies. The shoots were initiated on MS medium supplemented with BAP (1-2 mg/litter). Further observations of multiple shoot proliferation by using different cytokinins are in progress. 170. Nutrient Optimization of Sugarcane in Periyar-Vaigai Command Area of Tamil Nadu S. Sheeba, A. Bhaskaran* and A. R. Mohamed Haroon Dept. of Soil and Environment, Agricultural College and Research Institute, Madurai Tamil Nadu Agricultural University, Sugarcane Breeding Institute, Coimbatore, Tamil Nadu Keywords : Sugarcane, Nutrients, Optimization, Maximum yield Sugarcane is one of the most important cash crops in India. It produces huge biomass and hence removes a substantial quantity of plant nutrients from the soil. Keeping this in view, Integrated Nutrient Management was adopted to avert the problem of nutrient mining in sugarcane growing areas and to optimize the major nutrients for sustainable sugarcane production. For optimizing N, P, K, Zn and Fe for sugarcane, a field experiment was conducted during 2005-2006 at P. C. Patty village of Theni district, with the variety Co 86032, by employing treatment combinations of four levels of N, P and K i. e., 0, 75, 100 and 125 per cent recommended dose of fertilizer without and with recommended level of ZnSO 4 , FeSO 4 , vermicompost and Azophos. Graded levels of NPK application with IPNM
134 Proc. 98th Indian Science Congress, Part II : Abstracts of Oral/Poster Presentation practices favourably influenced the cane yield. The yield obtained ranged from 76. 5 to 142 t ha -1 . The highest cane yield (142 t ha -1 ) was recorded in the treatment that received N @ 340 kg ha -1 along with the recommended dose of P, K, ZnSO 4 , FeSO 4 , vermicompost and Azophos. Biometric characteristics viz., number of millable cane, length and girth of millable cane, number of internodes and length of internodes in millable cane were higher in 340 kg N ha -1 application which was on par with recommended dose of N (275 kg ha -1 ). The N, P, K, Zn and Fe contents increased with increasing levels of N, P and K along with recommended dose of ZnSO 4 , FeSO 4 , vermicompost and Azophos. With the advancement of crop growth stages, the nutrient content decreased progressively. The major fertilizer nutrient levels were optimized using Mitcherlich – Bray equation and targeted yield concept. The maximum yield (A) of 160 t ha -1 was arrived through least square function using the yield data. N, P and K requirement at different soil test values for achieving the target yield of 155 t ha -1 was yield maximum (A) was 160 t ha -1 . By using the Mitcherlich-Bray equation, physical and economic optimum of N, P and K were computed and the results revealed that the applied N and K levels were found to be equal to that of recommended dose. However, it seems to be higher for P as compared to recommended dose. The fertilizer recommendations by fertilizer prescription equation for 155 t ha -1 of yield target were found to be more than that of blanket fertilizer recommendation. 171. Effect of Sucrose and Chlorocholine Chloride on in Vitro Production of Microtubers in the Potato Cultivars Raghavendra Aminedi, Hitaishi Khandal and Niranjan Das* Deptt. of Biotechnology & Environmental Sciences, Thapar University, Patiala-147004 Keywords : Potato, microtuber, sucrose, chlorocholine chloride (CCC) Production of potato microtubers in vitro is an important aspect of crop biotechnology. As reported in the literature, media formulations usually include 8%
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