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INDIAN SCIENCE CONGRESS - India Environment Portal

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Section XIV : Plant Sciences 9<br />

Asparaginase is an important enzyme used as an anti cancer compound. Salt<br />

marsh plant (Suveda monoica) was used for fungal isolation by triple surface<br />

sterilization method. Twenty three isolates of endophytic fungi were evaluated for<br />

asparaginase production using qualitative and quantitative analyses. Seven isolates<br />

demonstrated pink zone around their colonies. Fusarium spp, and Aspergillus spp,<br />

isolated from salt marsh plant were highly active when tested with quantitative<br />

assay. The endophytic fungi with high activity are proposed as a possible asparaginase<br />

source to manage cancer cells.<br />

11. Phytotoxic Effects of Pyrethroid Insecticide on Seed Germination of<br />

Zea mays<br />

Shraddha Sharma and Babita Sharma<br />

Institute of Applied Medicines & Research<br />

9th Km. Stone, Delhi-Meerut Road, Duhai,<br />

Ghaziabad (U.P.)<br />

Keywords : Zea mays cypermethrin,<br />

For the present study Zea mays var. Ganga 2 was selected. The insecticide<br />

chosen for the study was cypermethrin (25% EC). For the study, three different<br />

concentrations of insecticide i. e. recommended dose 0. 05% (x), twice the<br />

recommended dose 0. 1% (2x) and four times the recommended dose 0. 2% (4x)<br />

were taken. The control was tap water. The uniform viable seeds of var. Ganga<br />

2 were soaked in various concentrations of insecticide and tap water. It was found<br />

that seed germination was inhibited at all the used concentrations. The seed vigour<br />

also declined as the concentrations of insecticide was increased from x to 4x.<br />

Various growth parameters like fresh weight and dry weight of the seedling, %<br />

moisture content, number of leaves, number of roots and average root lengths were<br />

adversely affected by the chemical stress induced by insecticide. The reduction in<br />

growth was minimum at the x dose and was maximum at 4x dose of insecticide.<br />

?-amylase activity also declined in response to pesticide treatment. Decrease in<br />

enzyme activity was maximum at 4x concentration. A decline in chlorophylls (a, b<br />

and total), carbohydrate, protein, carotenoid and anthocyanin content was observed<br />

in the seedlings exposed to different concentrations of insecticide.

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