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BIOTECHNOLOGY - IE

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Topic 5: Expression vectors<br />

Session Nr: 4 (theory)<br />

Contents:<br />

- Prokaryotes expression systems.<br />

- Fusion proteins.<br />

- Cell cultures<br />

Competencies:<br />

- Know the different expression systems and understand how they work.<br />

- Understand the concept of fusion proteins and their applications.<br />

Module II: Biotechnology applications.<br />

Topic 6: Gene therapy<br />

Session Nr: 5 (theory)<br />

Contents:<br />

- In vivo and ex vivo gene therapy.<br />

- Vectors.<br />

Competencies:<br />

- Be aware of the use of normal genes to supplement or replace defective ones or to<br />

bolster a normal function such as immunity.<br />

Topic 7: Genetic engineering applications to the detection and diagnostics systems.<br />

Session Nr: 6 (theory) 7 and 8 (Lab)<br />

Contents:<br />

- Immunologic diagnostics.<br />

- Monoclonal antibodies.<br />

- Prionics test.<br />

- Coliclonals antibodies.<br />

- Molecular diagnostics.<br />

- DNA fingerprint: applications to the forensic medicine.<br />

- DNA chips and Microarrays: applications.<br />

- Biosensors: types and applications.<br />

Competencies:<br />

- Be aware of the main genetic engineering techniques and understand their applications to<br />

the detection and diagnostic systems.<br />

4⏐

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