Developing 384-plex SNP marker sets for breeding and ... - icrisat
Developing 384-plex SNP marker sets for breeding and ... - icrisat
Developing 384-plex SNP marker sets for breeding and ... - icrisat
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<strong>SNP</strong> versus SSR throughput<br />
Manual PAGE genotyping:<br />
2 or 3 researchers<br />
BeadXpress genotyping:<br />
1 researcher<br />
16 PCR plates per day<br />
(8 gels morning, 8 afternoon)<br />
8 plates = 768 genotypes/day<br />
16 plates = 1536 genotypes/day<br />
QTL study: 288 lines x 100 SSRs<br />
= 300 PCR plates = 19 days<br />
96 x <strong>384</strong> <strong>SNP</strong>s in 2<br />
days = 18,432<br />
genotypes/day<br />
QTL study: 288 lines x<br />
<strong>384</strong> <strong>SNP</strong>s = 6 days
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