Thesis-PDF - IAP/TU Wien

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Chapter 8 Acknowledgments This work would not have been possible without the great support of a number of people. I would like to heartily thank Ille Gebeshuber for showing me wonderful science, for her great enthusiasm and all the long hours reading and correcting the manuscript, Friedrich Aumayr for his patience as well as for his encouragement and the constructive criticism regarding my work, Herbert Stachelberger for helping me with fluorescence microscopy at the Institut für Verfahrenstechnik and for sharing his motivation and enthusiasm, Paolo Gualtieri, Laura Barsanti and their group at the Institute of Biophysics at the CNR in Pisa, Italy, for introducing me to Euglena gracilis and related laboratory techniques as well as providing samples for my AFM investigations, the members of the Arbeitsgruppe "Atomic and Plasma Physics" at the Institut für Allgemeine Physik, especially Michael Brunmayr, Christoph Gösselsberger, Oliver Hekele, Donat Holzer, Katharina Igenbergs, Cezary Pieczetowski and Robert Ritter for fruitful discussions and a cheerful time, the friendly and supportive staff at the Institute für Allgemeine Physik, making it a pleasure to work there, my family for continuous support, Rosika for motivating me to get things done, & all the others who were there when I needed them. 94
Appendix A Acronyms SPM Scanning Probe Microscope/Microscopy STM Scanning Tunneling Microscope/Microscopy TEM Transmission Electron Microscope/Microscopy SEM Scanning Electron Microscope/Microscopy AFM Atomic Force Microscope/Microscopy 3D three-dimensional DC direct current NASA National Aeronautics and Space Association MFM Magnetic Force Microscope/Microscopy UHV Ultra High Vacuum 95
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DIPLOMARBEIT Atomic Force Microscop
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»Later I have been asked many time
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Contents Abstract 4 1 Introduction
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5.2.1 Optical Microscopy and Fluore
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theory (see e.g. [1]). Now I call o
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understand phenomena at a larger sc
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of whole dried Euglena cells in air
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these assembly functions for us. 2
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Figure 2.2: Rise in public spending
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The presented three types of nanote
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2.4.1 Self-Assembly and Emergent St
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After diligent investigation such s
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1 µN) between a probe and the samp
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3.2.1 Static Mode In Static Mode (a
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crystal and the cantilever tip (pha
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Figure 3.6: Highly ordered and fres
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Figure 3.8: Schematic drawing of th
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Figure 3.11: The cantilever deflect
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Figure 3.14: This force curve shows
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Figure 4.1: A group of Euglena orga
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several species with rigid pellicle
Page 43 and 44: Figure 4.2: The rightmost cell can
Page 45 and 46: cavity and is used for regulatory f
Page 47 and 48: as phobic reactions, because the ce
Page 49 and 50: Figure 4.7: Scheme of the photorece
Page 51 and 52: Figure 4.9: Measured monoclinic uni
Page 53 and 54: Figure 4.11: Twice the same Euglena
Page 55 and 56: 4.15), their ends are termed the (-
Page 57 and 58: Figure 4.15: Kinesin and dynein and
Page 59 and 60: Figure 4.17: (Image reproduced from
Page 61 and 62: into fully functional chloroplasts
Page 63 and 64: Figure 4.19: How a microtubule-kine
Page 65 and 66: Chapter 5 Materials and Methods The
Page 67 and 68: Cell Treatment - Demembranation In
Page 69 and 70: Figure 5.3: This image shows cells
Page 71 and 72: Figure 5.4: The AFM setup at the In
Page 73 and 74: Figure 5.6: If the user menu AFM_TU
Page 75 and 76: Figure 5.7: Single steps of the sam
Page 77 and 78: Chapter 6 Results High resolution t
Page 79 and 80: Figure 6.2: An embedded E. gracilis
Page 81 and 82: Figure 6.5: Scanning under liquid s
Page 83 and 84: Flagellum The flagellum of an E. gr
Page 85 and 86: Pellicle The pellicle of E. gracili
Page 87 and 88: Figure 6.11: The pellicle of an E.
Page 89 and 90: Crystalline Cell Parts Crystalline
Page 91 and 92: Figure 6.18: A layered structure th
Page 93: It might also be possible that comb
Page 97 and 98: [13] A. Junk, F. Riess: From an ide
Page 99 and 100: [45] J. Ruan, B. Bhushan: Atomic-sc
Page 101 and 102: [70] M. Rief, M. Gautel, F. Oesterh
Page 103 and 104: [97] P. Gualtieri, L. Barsanti: Alg
Page 105 and 106: Annex 1 This Igor script was used i
Page 107 and 108: w[i][j][laynr]=(w[i][j][3]-used_off
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