Thesis-PDF - IAP/TU Wien

More documents

Recommendations

Info

Figure 6.8: Left: Detail view of a part of an E. gracilis flagellum, completely covered by mastigonemes (fine hairlike structures). Intermittent contact mode AFM image, Amplitude trace. Right: Diagram illustrating a possible arrangement of mastigonemes along the flagellar surface. Image adapted from [82]. 84
Pellicle The pellicle of E. gracilis could be imaged with AFM with excellent resolution and detail. For example in Fig. 6.9 the apical part of such a cell is shown, revealing the flagellum, a deepened shape of the reservoir and an embossed shape of a crystalline storage grain (paramylum). The visibility of the reservoir and the paramylum grain results from the flattening of the cell due to the drying process where the algal pellicle sinks into the reservoir and relaxes over the hard paramylum grain. In Fig. 6.10, the middle part of an E. gracilis, the course of the pellicular strips is very distinct. They are arranged diagonally and are very uniform in thickness and direction. The pellicle usually grows as a left-handed spiral. Some paramylums show as cameo shapes in the pellicle. Figure 6.9: Apical part of an E. gracilis cell. a flagellum, b reservoir, c cameo shape of a paramylum grain. Image size 20 × 20 µm 2 , Intermittent contact mode AFM image, Amplitude trace. Zooming into the details of a typical pellicle of an E. gracilis cell, the articulations between the strips can be seen showing lubricant material features. In Fig. 6.11, a detail view of a group of pellicle strips is given. The small spots distributed mainly between the strips are mucilage, biological lubricant produced by the algae. The alga is able to actively slide its pellicle strips against each other in order to change its shape. A high resolution detail of previous figure is given in Fig. 6.12 that shows again the mucilage between the strips as well as microtubuli that presumably stem from another disrupted cell. Euglenoid mucilage, a polysaccharide network specially 85
Page 1 and 2:
DIPLOMARBEIT Atomic Force Microscop
Page 3 and 4:
»Later I have been asked many time
Page 5 and 6:
Contents Abstract 4 1 Introduction
Page 7 and 8:
5.2.1 Optical Microscopy and Fluore
Page 9 and 10:
theory (see e.g. [1]). Now I call o
Page 11 and 12:
understand phenomena at a larger sc
Page 13 and 14:
of whole dried Euglena cells in air
Page 15 and 16:
these assembly functions for us. 2
Page 17 and 18:
Figure 2.2: Rise in public spending
Page 19 and 20:
The presented three types of nanote
Page 21 and 22:
2.4.1 Self-Assembly and Emergent St
Page 23 and 24:
After diligent investigation such s
Page 25 and 26:
1 µN) between a probe and the samp
Page 27 and 28:
3.2.1 Static Mode In Static Mode (a
Page 29 and 30:
crystal and the cantilever tip (pha
Page 31 and 32:
Figure 3.6: Highly ordered and fres
Page 33 and 34: Figure 3.8: Schematic drawing of th
Page 35 and 36: Figure 3.11: The cantilever deflect
Page 37 and 38: Figure 3.14: This force curve shows
Page 39 and 40: Figure 4.1: A group of Euglena orga
Page 41 and 42: several species with rigid pellicle
Page 43 and 44: Figure 4.2: The rightmost cell can
Page 45 and 46: cavity and is used for regulatory f
Page 47 and 48: as phobic reactions, because the ce
Page 49 and 50: Figure 4.7: Scheme of the photorece
Page 51 and 52: Figure 4.9: Measured monoclinic uni
Page 53 and 54: Figure 4.11: Twice the same Euglena
Page 55 and 56: 4.15), their ends are termed the (-
Page 57 and 58: Figure 4.15: Kinesin and dynein and
Page 59 and 60: Figure 4.17: (Image reproduced from
Page 61 and 62: into fully functional chloroplasts
Page 63 and 64: Figure 4.19: How a microtubule-kine
Page 65 and 66: Chapter 5 Materials and Methods The
Page 67 and 68: Cell Treatment - Demembranation In
Page 69 and 70: Figure 5.3: This image shows cells
Page 71 and 72: Figure 5.4: The AFM setup at the In
Page 73 and 74: Figure 5.6: If the user menu AFM_TU
Page 75 and 76: Figure 5.7: Single steps of the sam
Page 77 and 78: Chapter 6 Results High resolution t
Page 79 and 80: Figure 6.2: An embedded E. gracilis
Page 81 and 82: Figure 6.5: Scanning under liquid s
Page 83: Flagellum The flagellum of an E. gr
Page 87 and 88: Figure 6.11: The pellicle of an E.
Page 89 and 90: Crystalline Cell Parts Crystalline
Page 91 and 92: Figure 6.18: A layered structure th
Page 93 and 94: It might also be possible that comb
Page 95 and 96: Appendix A Acronyms SPM Scanning Pr
Page 97 and 98: [13] A. Junk, F. Riess: From an ide
Page 99 and 100: [45] J. Ruan, B. Bhushan: Atomic-sc
Page 101 and 102: [70] M. Rief, M. Gautel, F. Oesterh
Page 103 and 104: [97] P. Gualtieri, L. Barsanti: Alg
Page 105 and 106: Annex 1 This Igor script was used i
Page 107 and 108: w[i][j][laynr]=(w[i][j][3]-used_off
show all

Thesis-PDF - IAP/TU Wien

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?