Thesis-PDF - IAP/TU Wien
Thesis-PDF - IAP/TU Wien
Thesis-PDF - IAP/TU Wien
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Figure 5.4: The AFM setup at the Institut für Allgemeine Physik at the<br />
<strong>TU</strong> Vienna. A - Scanning Head, B - Scanning Stage, C - Top View Optics,<br />
D - Top View Optics Light Source, E - Zeiss Inverted Optical Microscope<br />
open the possibility to implement new measurement techniques. It enables real<br />
time control of this type of AFM and possesses data acquisition functions, offline<br />
analysis and data manipulation tools. ([69])<br />
In order to ease the analysis of measured data, an Igor script was written,<br />
helping to reduce image artifacts resulting from the sometimes slow feedback loop.<br />
Only the combination of trace and retrace image data (i.e. scanning along the<br />
x-axis in both directions) gives the full information about sample topography (see<br />
Fig. 5.5).<br />
This calculation for the combination of trace and retrace images (for height or<br />
amplitude information) was included as as Igor script and made accessible through<br />
a custom menu (See Fig. 5.6 and Annex 1 on page 105 for the script file).<br />
5.3 Sample preparation<br />
5.3.1 Slide preparation<br />
Well prepared slides are a prerequisite for meaningful optical and fluorescence<br />
microscopy. Cell preparation includes the collection by centrifugation, treatment<br />
of the cell, immobilizing of cells by applying the proper pressure on the thin glass<br />
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