Thesis-PDF - IAP/TU Wien

Figure 5.4: The AFM setup at the Institut für Allgemeine Physik at the
TU Vienna. A - Scanning Head, B - Scanning Stage, C - Top View Optics,
D - Top View Optics Light Source, E - Zeiss Inverted Optical Microscope
open the possibility to implement new measurement techniques. It enables real
time control of this type of AFM and possesses data acquisition functions, offline
analysis and data manipulation tools. ([69])
In order to ease the analysis of measured data, an Igor script was written,
helping to reduce image artifacts resulting from the sometimes slow feedback loop.
Only the combination of trace and retrace image data (i.e. scanning along the
x-axis in both directions) gives the full information about sample topography (see
Fig. 5.5).
This calculation for the combination of trace and retrace images (for height or
amplitude information) was included as as Igor script and made accessible through
a custom menu (See Fig. 5.6 and Annex 1 on page 105 for the script file).
5.3 Sample preparation
5.3.1 Slide preparation
Well prepared slides are a prerequisite for meaningful optical and fluorescence
microscopy. Cell preparation includes the collection by centrifugation, treatment
of the cell, immobilizing of cells by applying the proper pressure on the thin glass
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