Thesis-PDF - IAP/TU Wien
Thesis-PDF - IAP/TU Wien
Thesis-PDF - IAP/TU Wien
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apply recognition force microscopy to living cells (e.g. [55]). The ligandreceptor<br />
binding is generally a reversible reaction. An advancement of above<br />
technique led to the possibility of simultaneous identification of two types of<br />
proteins in compositionally complex samples. ([56])<br />
- Recognition imaging combines molecular recognition with surface imaging.<br />
It means the mapping of ligand-receptor interaction sites on surfaces<br />
with nanometer positional accuracy. E.g. identification and localization of<br />
receptor binding sites distributed on biological membranes.<br />
- Real Time Protein-Protein Interactions ([57])<br />
Figure 3.7: The differentiation of atomic species by AFM as achieved<br />
through measurement of short-range forces. Atoms of the same type are<br />
colored the same. ([47])<br />
3.3.2 Atomic Collection Information<br />
- Surface topography<br />
- Surface profiling<br />
- Measurement of adhesion and friction of solid and liquid surfaces. This<br />
can be achieved through measurement of lateral forces on the cantilever<br />
tip. Any AFM that can measure the tilt (see Fig. 3.9) of its scanning<br />
cantilever, e.g. with a four-quadrant photodiode, can perform lateral force<br />
measurements. Lateral force microscopy (LFM) measures lateral deflections<br />
(tilt) of the cantilever arising from forces on its tip parallel to the plane of<br />
the sample surface. (ref. [58], [59], [60], [61], [62])<br />
- Magnetic Force Microscopy (MFM). With an AFM cantilever, that is<br />
coated with a ferromagnetic thin film, the spatial variation of magnetic forces<br />
on a sample surface can be imaged. The tip is hovered over the surface in<br />
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