Thesis-PDF - IAP/TU Wien

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them captive. Later Leonardo da Vinci fills whole notebooks with his sketches of bird flight maneuvers, making detailed observations of how birds execute turns, noting their wing and tail postures. He used these then to devise novel concepts for man-carrying machines (see Fig. 1.2) and devices for the testing of wings. Then Figure 1.2: To the left a part of a flight machine sketch of Leonardo da Vinci. To the right a bird wing study of Otto Lilienthal. Images adapted from [9] other inventors like Sir George Cayley (1773-1857), Otto Lilienthal (1848-96) and the Wright brothers Orville and Wilbur (1871–1948, 1867–1912), also deduced key principles of flight from their studies of birds ([10]). The winglet, an inspiration drawn from bird flight, was already invented in 1897. It can be described as small fin sticking straight up on the wingtip of many modern airplanes and considerably helps reducing fuel consumption ([11]). This work investigates such a promising system - the alga Euglena gracilis, exhibiting many properties with high potential interest for nanotechnological applications. How nanotechnology can be characterized and some of its main aspects are discussed in the second chapter (3). The third chapter (3) deals with Atomic Force Microscopy (AFM). Techniques are explained and classified, with a focus on the application to biological systems. The biological system Euglena gracilis is investigated in chapter 4. The description focuses on its morphology in respect to structure, organization and function. Subsystems of the alga are evaluated regarding their potential as templates and idea supply for man-made nanotechnological applications. The experimental part of this work is divided into two chapters. The setup of the experiments as well as the methods of investigation used is presented in chapter 5. This includes how the algal cultures had to be kept under laboratory conditions, how they were collected, treated and prepared and how the AFM investigations were carried out. A preparation method for atomic force microscopy investigation 12
of whole dried Euglena cells in air was developed and provided data on whole cells as well as cell organelles. In the results chapter 6, obtained image data is presented and compared to already available Transmission and Scanning Electron Microscopy data. Compared to traditional imaging approaches of biological samples, AFM can be used as a nondestructive imaging method. New features on the pellicle not seen by former TEM and SEM studies were found and questioned. Finally, the outlook chapter sketches out possible directions of future research. The need for an interdisciplinary approach when investigating or conceiving nanosystems is apparent. The consequences of nanotechnology and bionanotechnology will reach everywhere in our daily lives - ethical, social and political consequences. But Science by itself never can solve the question, how we can responsibly use our knowledge and abilities to the benefit of mankind. Such questions must be posed as early as possible, in order to be openly addressed and discussed. 13
Page 1 and 2: DIPLOMARBEIT Atomic Force Microscop
Page 3 and 4: »Later I have been asked many time
Page 5 and 6: Contents Abstract 4 1 Introduction
Page 7 and 8: 5.2.1 Optical Microscopy and Fluore
Page 9 and 10: theory (see e.g. [1]). Now I call o
Page 11: understand phenomena at a larger sc
Page 15 and 16: these assembly functions for us. 2
Page 17 and 18: Figure 2.2: Rise in public spending
Page 19 and 20: The presented three types of nanote
Page 21 and 22: 2.4.1 Self-Assembly and Emergent St
Page 23 and 24: After diligent investigation such s
Page 25 and 26: 1 µN) between a probe and the samp
Page 27 and 28: 3.2.1 Static Mode In Static Mode (a
Page 29 and 30: crystal and the cantilever tip (pha
Page 31 and 32: Figure 3.6: Highly ordered and fres
Page 33 and 34: Figure 3.8: Schematic drawing of th
Page 35 and 36: Figure 3.11: The cantilever deflect
Page 37 and 38: Figure 3.14: This force curve shows
Page 39 and 40: Figure 4.1: A group of Euglena orga
Page 41 and 42: several species with rigid pellicle
Page 43 and 44: Figure 4.2: The rightmost cell can
Page 45 and 46: cavity and is used for regulatory f
Page 47 and 48: as phobic reactions, because the ce
Page 49 and 50: Figure 4.7: Scheme of the photorece
Page 51 and 52: Figure 4.9: Measured monoclinic uni
Page 53 and 54: Figure 4.11: Twice the same Euglena
Page 55 and 56: 4.15), their ends are termed the (-
Page 57 and 58: Figure 4.15: Kinesin and dynein and
Page 59 and 60: Figure 4.17: (Image reproduced from
Page 61 and 62: into fully functional chloroplasts
Page 63 and 64:
Figure 4.19: How a microtubule-kine
Page 65 and 66:
Chapter 5 Materials and Methods The
Page 67 and 68:
Cell Treatment - Demembranation In
Page 69 and 70:
Figure 5.3: This image shows cells
Page 71 and 72:
Figure 5.4: The AFM setup at the In
Page 73 and 74:
Figure 5.6: If the user menu AFM_TU
Page 75 and 76:
Figure 5.7: Single steps of the sam
Page 77 and 78:
Chapter 6 Results High resolution t
Page 79 and 80:
Figure 6.2: An embedded E. gracilis
Page 81 and 82:
Figure 6.5: Scanning under liquid s
Page 83 and 84:
Flagellum The flagellum of an E. gr
Page 85 and 86:
Pellicle The pellicle of E. gracili
Page 87 and 88:
Figure 6.11: The pellicle of an E.
Page 89 and 90:
Crystalline Cell Parts Crystalline
Page 91 and 92:
Figure 6.18: A layered structure th
Page 93 and 94:
It might also be possible that comb
Page 95 and 96:
Appendix A Acronyms SPM Scanning Pr
Page 97 and 98:
[13] A. Junk, F. Riess: From an ide
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[45] J. Ruan, B. Bhushan: Atomic-sc
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[70] M. Rief, M. Gautel, F. Oesterh
Page 103 and 104:
[97] P. Gualtieri, L. Barsanti: Alg
Page 105 and 106:
Annex 1 This Igor script was used i
Page 107 and 108:
w[i][j][laynr]=(w[i][j][3]-used_off
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