Thesis-PDF - IAP/TU Wien

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function join_amptampr (w) // joining amplitude trace and retrace Wave w Variable laynr Variable i Variable j Variable offset=0 Variable used_offset Variable test Variable test1 Variable separationline=0.00000005 // used to distinguish from which area information comes Variable sep=0 // if 1: active Variable fuzz=0.2 // 0: offset to be used: mean-offset, // 1: dot-per-dot-offset, or use value in between print ("separation line height if present is:"), separationline print ("fuzz parameter is:"), fuzz laynr = DimSize(w, 2) // number of layers Redimension/N=(-1,-1,laynr+1) w // create a new layer for(i=0;i< DimSize(w, 0);i+=1) // initialize variables;continue test for(j=0;j< DimSize(w, 1);j+=1) offset = offset+w[i][j][2]+w[i][j][3] endfor // condition;update loop variables endfor //calculating mean offset for amplitude traces offset=offset/(2*DimSize(w, 0)*DimSize(w,1)) print ("mean offset is:"), offset for(i=0;i< DimSize(w, 0);i+=1) for(j=0;j< DimSize(w, 1);j+=1) // initialize variables;continue test used_offset = offset*(1-fuzz) +fuzz*(w[i][j][2]+w[i][j][3])/2 if (w[i][j][0]==min(w[i][j][0],w[i][j][1])) w[i][j][laynr]=w[i][j][2]-used_offset //taken from amplTRACE if corresponding heightTRACE is min test=0 else 106
w[i][j][laynr]=(w[i][j][3]-used_offset)*(-1) // taken from amplRETRACE if corresponding heightRETRACE is min test=1 endif if (test1!=test && sep==1) w[i][j][laynr]=separationline endif test1=test endfor endfor // condition;update loop variables // execute body code until continue test is FALSE end // change LayerLabel so that the MFP-3D extension displays the new layer SetDimLabel 2, laynr, UserIn2Trace, w function NewLayer() String workwave Variable op_number Variable i SetDataFolder root:images // generate list of afm data waves String wlist = SortList(WaveList("*0*", ";", "")) Prompt workwave, "Select Wave", popup wlist Prompt op_number, "Select New Layer Content", popup "Join Height; Lateral T - R;Join Amplitude;HeightnAmp for all;Delete Layer;" DoPrompt "Make Layer and calculate!", workwave, op_number if ( V_flag ) return -1 // user canceled endif switch(op_number) // numeric switch case 1: // execute if case matches expression min_htthtr($workwave) break // exit from switch case 2: LateralTR($workwave) break 107
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DIPLOMARBEIT Atomic Force Microscop
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»Later I have been asked many time
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Contents Abstract 4 1 Introduction
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5.2.1 Optical Microscopy and Fluore
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theory (see e.g. [1]). Now I call o
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understand phenomena at a larger sc
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of whole dried Euglena cells in air
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these assembly functions for us. 2
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Figure 2.2: Rise in public spending
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The presented three types of nanote
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2.4.1 Self-Assembly and Emergent St
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After diligent investigation such s
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1 µN) between a probe and the samp
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3.2.1 Static Mode In Static Mode (a
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crystal and the cantilever tip (pha
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Figure 3.6: Highly ordered and fres
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Figure 3.8: Schematic drawing of th
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Figure 3.11: The cantilever deflect
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Figure 3.14: This force curve shows
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Figure 4.1: A group of Euglena orga
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several species with rigid pellicle
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Figure 4.2: The rightmost cell can
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cavity and is used for regulatory f
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as phobic reactions, because the ce
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Figure 4.7: Scheme of the photorece
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Figure 4.9: Measured monoclinic uni
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Figure 4.11: Twice the same Euglena
Page 55 and 56: 4.15), their ends are termed the (-
Page 57 and 58: Figure 4.15: Kinesin and dynein and
Page 59 and 60: Figure 4.17: (Image reproduced from
Page 61 and 62: into fully functional chloroplasts
Page 63 and 64: Figure 4.19: How a microtubule-kine
Page 65 and 66: Chapter 5 Materials and Methods The
Page 67 and 68: Cell Treatment - Demembranation In
Page 69 and 70: Figure 5.3: This image shows cells
Page 71 and 72: Figure 5.4: The AFM setup at the In
Page 73 and 74: Figure 5.6: If the user menu AFM_TU
Page 75 and 76: Figure 5.7: Single steps of the sam
Page 77 and 78: Chapter 6 Results High resolution t
Page 79 and 80: Figure 6.2: An embedded E. gracilis
Page 81 and 82: Figure 6.5: Scanning under liquid s
Page 83 and 84: Flagellum The flagellum of an E. gr
Page 85 and 86: Pellicle The pellicle of E. gracili
Page 87 and 88: Figure 6.11: The pellicle of an E.
Page 89 and 90: Crystalline Cell Parts Crystalline
Page 91 and 92: Figure 6.18: A layered structure th
Page 93 and 94: It might also be possible that comb
Page 95 and 96: Appendix A Acronyms SPM Scanning Pr
Page 97 and 98: [13] A. Junk, F. Riess: From an ide
Page 99 and 100: [45] J. Ruan, B. Bhushan: Atomic-sc
Page 101 and 102: [70] M. Rief, M. Gautel, F. Oesterh
Page 103 and 104: [97] P. Gualtieri, L. Barsanti: Alg
Page 105: Annex 1 This Igor script was used i
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