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[112] T. Maddanimatha, G. Ramanath, K. Vijayamohanan: Self-assembled molecular nanocathodes for rechargeable energy storage modules, Chem, Phys. Let. 396/4-6, (2004), pp. 277-281 [113] D. Bäumer, A. Preisfeld, H. G. Ruppel: Isolation and Characterization of Paramylon Synthase from Euglena gracilis (Euglenophyceae), Journ. Phycology 37/1, (2001), pp. 38-46 [114] M. Cramer, J. Myers: Growth and photosynthetic characteristics of Euglena gracilis, Arch. Microbiol. 17, pp. 384-402 [115] L. Barsanti, V. Passarelli, P. L. Walne, P. Gualtieri: The photoreceptor protein of Euglena gracilis, FEBS Letters, 482 (2000), p. 247 [116] H. J. Arnott, P. L. Walne: Observations on the fine structure of the pellicle pores of Euglena granulata, Protoplasma 64 (1967), p. 330 [117] C. Sanchez, H. Arribart, M. M. Giraud Guille: Biomimetism and bioinspiration as tools for the designs of innovative materials and systems, Nature materials, 4 (2005), p. 277 [118] B. S. Leander, M. A. Farmer: Comparative morphology of the euglenid pellicle. I. Patterns of strips and pores, J. Eukaryot. Microbiol. 47 (2000), p. 469 [119] C. Gruenberger, R. Ritter, F. Aumayr, H. Stachelberger, I. C. Gebeshuber: Algal biophysics: Euglena gracilis investigated by atomic force microscopy, Materials Science Forum Vol. 555 (2007), pp. 411-416 104
Annex 1 This Igor script was used in order to remove image artifacts from acquired image data as described in 5.2.3 under AFM Software and Fig. 5.5 (page 70 and 72 respectively). #pragma rtGlobals = 1 // Use modern global access method. menu "AFM_<strong>TU</strong>VIENNA" // add new entry into menu. "TraceRetrace Calculations... " , NewLayer() end function LateralTR (w) // function to add a new layer to an existing afm data wave Wave w Variable laynr end laynr = DimSize(w, 2) //number of layers Redimension/N=(-1,-1,laynr+1) w // create a new layer w[][][laynr] = w[p][q][laynr -2] - w[p][q][laynr -1] // calculate the lateral T - R signal // change LayerLabel so that the MFP-3D extension displays the new layer SetDimLabel 2, laynr, UserIn0Trace, w function min_htthtr (w) // function joining height trace and retrace Wave w Variable laynr end laynr = DimSize(w, 2) // get the number of layers Redimension/N=(-1,-1,laynr+1) w // create a new layer w[][][laynr]=min(w[p][q][0],w[p][q][1]) // change LayerLabel so that the MFP-3D extension displays the new layer SetDimLabel 2, laynr, UserIn1Trace, w 105
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DIPLOMARBEIT Atomic Force Microscop
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»Later I have been asked many time
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Contents Abstract 4 1 Introduction
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5.2.1 Optical Microscopy and Fluore
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theory (see e.g. [1]). Now I call o
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understand phenomena at a larger sc
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of whole dried Euglena cells in air
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these assembly functions for us. 2
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Figure 2.2: Rise in public spending
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The presented three types of nanote
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2.4.1 Self-Assembly and Emergent St
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After diligent investigation such s
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1 µN) between a probe and the samp
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3.2.1 Static Mode In Static Mode (a
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crystal and the cantilever tip (pha
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Figure 3.6: Highly ordered and fres
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Figure 3.8: Schematic drawing of th
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Figure 3.11: The cantilever deflect
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Figure 3.14: This force curve shows
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Figure 4.1: A group of Euglena orga
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several species with rigid pellicle
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Figure 4.2: The rightmost cell can
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cavity and is used for regulatory f
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as phobic reactions, because the ce
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Figure 4.7: Scheme of the photorece
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Figure 4.9: Measured monoclinic uni
Page 53 and 54: Figure 4.11: Twice the same Euglena
Page 55 and 56: 4.15), their ends are termed the (-
Page 57 and 58: Figure 4.15: Kinesin and dynein and
Page 59 and 60: Figure 4.17: (Image reproduced from
Page 61 and 62: into fully functional chloroplasts
Page 63 and 64: Figure 4.19: How a microtubule-kine
Page 65 and 66: Chapter 5 Materials and Methods The
Page 67 and 68: Cell Treatment - Demembranation In
Page 69 and 70: Figure 5.3: This image shows cells
Page 71 and 72: Figure 5.4: The AFM setup at the In
Page 73 and 74: Figure 5.6: If the user menu AFM_TU
Page 75 and 76: Figure 5.7: Single steps of the sam
Page 77 and 78: Chapter 6 Results High resolution t
Page 79 and 80: Figure 6.2: An embedded E. gracilis
Page 81 and 82: Figure 6.5: Scanning under liquid s
Page 83 and 84: Flagellum The flagellum of an E. gr
Page 85 and 86: Pellicle The pellicle of E. gracili
Page 87 and 88: Figure 6.11: The pellicle of an E.
Page 89 and 90: Crystalline Cell Parts Crystalline
Page 91 and 92: Figure 6.18: A layered structure th
Page 93 and 94: It might also be possible that comb
Page 95 and 96: Appendix A Acronyms SPM Scanning Pr
Page 97 and 98: [13] A. Junk, F. Riess: From an ide
Page 99 and 100: [45] J. Ruan, B. Bhushan: Atomic-sc
Page 101 and 102: [70] M. Rief, M. Gautel, F. Oesterh
Page 103: [97] P. Gualtieri, L. Barsanti: Alg
Page 107 and 108: w[i][j][laynr]=(w[i][j][3]-used_off
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