LIFE09200604002 Lalit Sehgal - Homi Bhabha National Institute

the desmosomal proteins recruitment to the cell border is dependent on the ATPase
activity of motor proteins. However, as ATPγS inhibits multiple cellular processes, these
results need to be confirmed with specific kinesin inhibitors.
Figure 4.3.24: ATPγS treatment inhibits the DSC2/3 recruitment to cell border. (A)
HCT116 cells were grown in low calcium medium for 20 hrs and then shifted to medium
with standard calcium levels in presence or absence of ATP analog ATPγS (500µM) for 2
hours. The immunoflouresence analysis was performed using specific antibodies to
DSC2/3 and α-Tubulin. Please note that DSC2/3 localizes to cell border at 60 min in
HCT116 cells treated with DMSO whereas DSC2/3 fails to localize to cell border in the
cells treated with ATPγS. No difference in α-tubulin staining was observed upon
treatment with ATPγS. (B) The mean fluorescence intensities at the cell borders were
measured for 24 cells in 3 independent experiments for each immunofluorescence
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