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Generation of knockdown mice that l
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STATEMENT BY AUTHOR This dissertati
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CERTIFICATE I certify that the thes
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I am thankful to Mr Uday Dandekar f
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3.16 Isolation of Genomic DNA (gDNA
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A synopsis of thesis to be submitte
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esults in loss of 14-3-3 binding an
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and NheI (NEB) to remove tetO-CMV,
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Hanging drop and wound healing assa
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360). To generate transgenic animal
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asement of the seminiferous tubules
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normal female, pups were screened f
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14-3-3ε only in the presence of HP
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proteins are expressed in testis (3
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5. Lalit Sehgal, Srikanth B., Khyat
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Presented a poster at 33rd All Indi
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shRNA siRNA TBS-T U.V WT C IF DSG D
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List of Figures: Page No 1. Figure
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37. Figure 4.3.20: 14-3-3γ interac
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CHAPTER 1 INTRODUCTION 39
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1.1.1 Cyclin Cdk complexes Eukaryot
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1.1.1.c Cyclin A cdk complexes The
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of which are associated with acquis
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1.2.2 The G1/S DNA Damage checkpoin
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- Page 51 and 52: cytoplasmic accumulation of cdc25 p
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- Page 55 and 56: G2 arrest and induced premature chr
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- Page 67 and 68: CHAPTER 2 AIMS AND OBJECTIVES 67
- Page 69 and 70: CHAPTER 3 MATERIALS AND METHODS 69
- Page 71 and 72: Name of oligonucleotide EF1α Forwa
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- Page 75 and 76: R E1 A GAACGAATAGTGAAGCCACAGATGTA s
- Page 77 and 78: 100mm 25ug 225ul 250ul 500 1000ul T
- Page 79 and 80: for the transgene were considered a
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- Page 83 and 84: mounting agent. Confocal images wer
- Page 85 and 86: 3.26 Antibodies used for Western bl
- Page 87 and 88: each plate was added cold 500ml of
- Page 89 and 90: 3.32 Hanging drop assay to determin
- Page 91 and 92: 3.37 Biodistribution of 18 F -FDG u
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- Page 97 and 98: Figure 4.1.1 Generation of lentivir
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- Page 107 and 108: Figure 4.2.1: Infection of morulae
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- Page 111 and 112: Figure 4.2.3: EGFP-f expression in
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- Page 115 and 116: 4.3 Generation of 14-3-3γ knockdow
- Page 117 and 118: numbering). Alignment was done usin
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- Page 121 and 122: the mouse tested infected with vect
- Page 123 and 124: Figure 4.3.7: Staging of 14-3-3γ k
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- Page 127 and 128: Figure 4.3.9: 14-3-3γ knockdown le
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- Page 131 and 132: intercellular space (12, 110, 115,
- Page 133 and 134: Figure 4.3.13: 14-3-3γ loss leads
- Page 135 and 136: Figure 4.3.16: Localization of PKP3
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- Page 141 and 142: from our laboratory demonstrated th
- Page 143 and 144: Figure 4.3.21: Plakoglobin recruitm
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KIF5B, however its reduction does n
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compared to vector control cells. (
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4.4 Generation of 14-3-3ε knockdow
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into the interstitial spaces of the
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Figure 4.4.3: 14-3-3ε knockdown in
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Figure 4.4.4: Patchy hair loss in 1
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follicle formation marked by black
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Figure 4.4.7: Phenotypes in 14-3-3
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compared to WT lungs (indicated by
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compared to wild type (Figure 4.4.1
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and CD4 positive lyumphocyte number
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Figure 4.4.13: Bone marrow cells fr
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Figure 4.4.15: Generation of stable
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Figure 4.4.16: NIH3T3 cells lacking
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Figure 4.4.18: Generation of induci
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CHAPTER 5 DISCUSSION 181
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to generate transgenic animals expr
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transgenic research and the use of
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(reviewed in (152, 374)). Kinesins
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subtypes known to date. Type IV col
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Figure 5.1 Schematic representation
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loss of 14-3-3ε the T cell activat
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BIBLIOGRAPHY: 1. Abraham, R. T. 200
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29. Blomer, U., L. Naldini, T. Kafr
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58. Cheng, C. Y., and D. D. Mruk. 2
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90. Elia, A. E., P. Rellos, L. F. H
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118. Girard, F., U. Strausfeld, J.
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150. Herrmann, H., M. Haner, M. Bre
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and activation of a cyclin E-cdk2 c
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213. Liu, D., J. Bienkowska, C. Pet
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242. Moll, R., W. W. Franke, D. L.
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274. Pagano, M., R. Pepperkok, F. V
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305. Russell, L. D. 1978. The blood
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inhibitor of metalloproteases-1 in
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364. Tsunekawa, N., M. Matsumoto, S
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397. Yashiro, M., N. Nishioka, and
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Research: Biology to Prevention to