LIFE01200604005 Shri Somnath Ghosh - Homi Bhabha National ...

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CHAPTER 2 MATERIALS AND METHODS PBS. Antibodies were diluted (1:200) in 1% BSA in PBS. Cells were incubated with primary antibodies for 1 h 30 min at room temperature, washed three times in PBS and incubated with secondary antibodies for 1 h at room temperature. Finally, cells were rinsed and mounted with ProLong Gold antifede with DAPI mounting media (Molecular Probe, USA). Images were captured using Carl Zeiss confocal microscope. Acquisition settings were optimized to obtain maximal signal in immunostained cells with minimal background. Table 2.6: The sequences of gene specific primers and their annealing temperature (Tm) used in RT-PCR experiments Gene Primer Sequence Tm ( o C) β-Actin F 5’-TGGAATCCTGTGGCATCCATGAAAC-3’ R 5’- TAAAACGCAGCTCAGTAACAGTCCG-3’ 55 iNOS F 5’- CTCTGACAGCCCAGAGTTCC - 3’ 62 R 5’- AGGCAAAGGAGGAGAAGGAG- 3’ p21 F 5’- GCCTTAGCCCTCACTCTGTG- 3’ 58 R 5’-GGTTGGGAGGGGCTTAAATA- 3’ p53 F 5’- CGGGTGGAAGGAAATTTGTA- 3’ 58 R 5’- CTTCTGTACGGCGGTCTCTC - 3’ p65 F 5’- ACAACTGAGCCCATGCTGAT- 3’ 50 ATM ATR R 5’- GAGAGGTCCATGTCCGCAAT- 3’ F 5’-GGACAGTGGAGGCACAAAAT-3’ R 5’-GTGTCGAAGACAGCTGGTGA-3’ F 5’-CTCGCTGAACTGTACGTGGA-3’ R 5’-GCATAGCTCGACCATGGATT-3’ 57 57 DNA-PK F 5’-TGCCAATCCAGCAGTCATTA-3’ 64 87
CHAPTER 2 MATERIALS AND METHODS Chk2 Chk1 Bax Bcl-2 Rad52 MLH1 CDKN1A (p21) GADD45α TLR3 FDXR R 5’-GGTCCATCAGGCACTTCACT-3’ F 5’-GGCTTCAGGATGAAGACATGA-3’ R 5’-CACAACACAGCAGCACACAC-3’ F 5’-TGTCAGAGTCTCCCAGTGGA-3’ R 5’-AGGGGCTGGTATCCCATAAG-3’ F 5’-TCCCCCCGAGAGGTCTTT-3’ R 5’-CGGCCCCAGTTGAAGTTG-3’ F 5’-GAGGATTGTGGCCTTCTTTG-3’ R 5’-ACAGTTCCACAAAGGCATCC-3’ F 5’-AGTTTTGGGAATGCACTTGG-3’ R 5’-TCGGCAGCTGTTGTATCTTG-3’ F 5’-GAGGTGAATTGGGACGAAGA-3’ R 5’-TCCAGGAGTTTGGAATGGAG-3’ F 5’-CAGCAGAGGAAGACCATGTG-3’ R 5’-GGCGTTTGGAGTGGTAGAAA-3’ F 5’-TGCGAGAACGACATCAACAT-3’ R 5’-TCCCGGCAAAAACAAATAAG-3’ F 5’-GCCTCTTCGTAACTTGACCA-3’ R 5’-AAGGATGTGGAGGTGAGACA-3’ F 5’-AGAGAACGGACATCACGAAG-3’ R 5’-GTCCTGGAGACCCAAGAAAT-3’ 64 59 56 59 50 52 59 58 57 57 F- Forward and R- Reverse 2.8 Image analysis using ImageJ software The captured images were analyzed for relative quantification of phosphorylation using ImageJ software [227]. A 25 x 25 pixel box was positioned over the fluorescent image of each cell, and 88
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RADIATION INDUCED SIGNALING IN MAMM
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DECLARATION I, hereby declare that
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CONTENTS Page No. SYNOPSIS………
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2.11 Measurement of NO production
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PREAMBLE SYNOPSIS Ionising radiatio
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SYNOPSIS Aims and Objectives: To St
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SYNOPSIS 2.6 Immunofluorescence sta
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SYNOPSIS ATM gene expression, which
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SYNOPSIS Percentage Survival 100 80
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SYNOPSIS Ratio of Rad52 to Actin Ra
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Relative Phosphorylation 45 40 35 3
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SYNOPSIS Fig. 3.3A Clonogenic Cell
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SYNOPSIS (A) Av No. of phospho BRCA
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SYNOPSIS Percentage Survival 120 10
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SYNOPSIS Fig.3.4B. Existance of cro
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SYNOPSIS Fig. 3.4EDNA damage in EL-
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SYNOPSIS subsequent cytotoxicity ca
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SYNOPSIS [4] Hall, E. J. Radiobiolo
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CHAPTER 1 INTRODUCTION INTRODUCTION
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CHAPTER 1 INTRODUCTION 15.8% 9.56%
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CHAPTER 1 INTRODUCTION far apart an
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CHAPTER 1 INTRODUCTION and are more
Page 46 and 47: CHAPTER 1 INTRODUCTION 1.3 DNA dama
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Page 50 and 51: CHAPTER 1 INTRODUCTION are unable t
Page 52 and 53: CHAPTER 1 INTRODUCTION Perhaps one
Page 54 and 55: CHAPTER 1 INTRODUCTION occurs at DS
Page 56 and 57: CHAPTER 1 INTRODUCTION related prot
Page 58 and 59: CHAPTER 1 INTRODUCTION damage must
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Page 70 and 71: CHAPTER 1 INTRODUCTION 1.4 Overview
Page 72 and 73: CHAPTER 1 INTRODUCTION 1.4.1 Radiat
Page 74 and 75: CHAPTER 1 INTRODUCTION interaction
Page 76 and 77: CHAPTER 1 INTRODUCTION p90S6 kinase
Page 78 and 79: CHAPTER 1 INTRODUCTION CD4 (formerl
Page 80 and 81: CHAPTER 1 INTRODUCTION unrepaired d
Page 82 and 83: CHAPTER 1 INTRODUCTION well to the
Page 84 and 85: CHAPTER 1 INTRODUCTION 1.6 Radiatio
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Page 88 and 89: CHAPTER 2 MATERIALS AND METHODS MAT
Page 90 and 91: CHAPTER 2 MATERIALS AND METHODS 2.2
Page 92 and 93: CHAPTER 2 MATERIALS AND METHODS res
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Page 100 and 101: CHAPTER 2 MATERIALS AND METHODS 2.1
Page 102 and 103: CHAPTER 3 RESULTS RESULTS 93
Page 104 and 105: CHAPTER 3 RESULTS fractionated regi
Page 106 and 107: CHAPTER 3 RESULTS On comparison of
Page 108 and 109: CHAPTER 3 RESULTS Table 3.1.2A List
Page 110 and 111: CHAPTER 3 RESULTS 80 NM_002185 IL7R
Page 112 and 113: CHAPTER 3 RESULTS SAA2 149 AU134977
Page 114 and 115: CHAPTER 3 RESULTS 221 LOC643167 RNA
Page 116 and 117: CHAPTER 3 RESULTS 299 BF244402 FBXO
Page 118 and 119: CHAPTER 3 RESULTS CDK4) 57 AU152107
Page 120 and 121: CHAPTER 3 RESULTS 134 AL045793 METT
Page 122 and 123: CHAPTER 3 RESULTS 58 AF237813 ABAT
Page 124 and 125: CHAPTER 3 RESULTS 127 AI809749 ORMD
Page 126 and 127: CHAPTER 3 RESULTS 31 BE615699 UNC84
Page 128 and 129: CHAPTER 3 RESULTS 47 AF026303 SULT1
Page 130 and 131: CHAPTER 3 RESULTS 117 BF062193 CYor
Page 132 and 133: CHAPTER 3 RESULTS 187 AL036662 ---
Page 134 and 135: CHAPTER 3 RESULTS 52 AV686514 EMP2
Page 136 and 137: CHAPTER 3 RESULTS exposed to 10 Gy
Page 138 and 139: CHAPTER 3 RESULTS Fig.3.1.4 Gene ex
Page 140 and 141: CHAPTER 3 RESULTS Fig. 3.1.6 Radiat
Page 142 and 143: CHAPTER 3 RESULTS Fig. 3.1.7 Transl
Page 144 and 145: CHAPTER 3 RESULTS 3.1.9 Stabilizati
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CHAPTER 3 RESULTS 23±1.5% (Fig. 3.
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CHAPTER 3 RESULTS whereas only the
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CHAPTER 3 RESULTS Fig.3.2.2 Gene ex
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CHAPTER 3 RESULTS Fig.3.2.4 Gene ex
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CHAPTER 3 RESULTS with equal doses
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CHAPTER 3 RESULTS Fig.3.3.1.2 Forma
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CHAPTER 3 RESULTS Fig.3.3.1.3 Phosp
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CHAPTER 3 RESULTS (7.5±0.64) (Fig.
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CHAPTER 3 RESULTS Fig.3.3.1.7b Phos
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CHAPTER 3 RESULTS 3.3.2 Oxygen beam
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CHAPTER 3 RESULTS in all the cells.
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CHAPTER 3 RESULTS 3.3.2.3 Radiation
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CHAPTER 3 RESULTS Fig.3.3.2.7 Apopt
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CHAPTER 3 RESULTS PMA stimulated U9
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CHAPTER 3 RESULTS Fig.3.4.2. Exista
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CHAPTER 3 RESULTS up-regulation of
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CHAPTER 3 RESULTS Fig.3.4.3.2 NO pr
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CHAPTER 3 RESULTS 3.4.3.4 Apoptotic
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CHAPTER 3 RESULTS 3.4.4 Bystander e
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CHAPTER 3 RESULTS Fig.3.4.4b DNA da
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CHAPTER 3 RESULTS Fig.3.4.5a Gene e
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CHAPTER 4 DISCUSSION DISCUSSION 185
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CHAPTER 4 DISCUSSION directly expos
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CHAPTER 4 DISCUSSION dose is delive
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CHAPTER 4 DISCUSSION A clear cause
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CHAPTER 4 DISCUSSION 4.2 Proton bea
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CHAPTER 4 DISCUSSION Although the e
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CHAPTER 4 DISCUSSION Similar number
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CHAPTER 4 DISCUSSION the mechanism
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CHAPTER 4 DISCUSSION Thus unlike th
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CHAPTER 4 DISCUSSION different from
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CHAPTER 4 DISCUSSION upregulates ma
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CHAPTER 4 DISCUSSION Numerous studi
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CHAPTER 4 DISCUSSION inhibition of
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CHAPTER 4 DISCUSSION The survival o
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CHAPTER 5 REFERENCES [1] Khan, F. T
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CHAPTER 5 REFERENCES [19] Woo, R. A
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CHAPTER 5 REFERENCES [35] Nghiem, P
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CHAPTER 5 REFERENCES [52] McKay, B.
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CHAPTER 5 REFERENCES [69] Cary, R.
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CHAPTER 5 REFERENCES [84] Uziel, T.
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CHAPTER 5 REFERENCES [98] Liu, Y.;
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CHAPTER 5 REFERENCES [111] Fei, P.;
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CHAPTER 5 REFERENCES [127] Frank, K
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CHAPTER 5 REFERENCES [141] Pierce,
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CHAPTER 5 REFERENCES [156] Roots, R
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CHAPTER 5 REFERENCES [171] Xia, Z.;
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CHAPTER 5 REFERENCES (MAP) kinase c
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CHAPTER 5 REFERENCES phosphorylatio
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CHAPTER 5 REFERENCES [211] Anderson
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CHAPTER 5 REFERENCES [229] Konca, K
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CHAPTER 5 REFERENCES [245] Kastan,
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CHAPTER 5 REFERENCES [261] Miralbel
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CHAPTER 5 REFERENCES [278] Weizman,
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CHAPTER 5 REFERENCES [294] Zhou, H.
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PUBLICATIONS AND PRESENTATIONS Publ
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Mutation Research 729 (2012) 61-72
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S. Ghosh, M. Krishna / Mutation Res
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Cancer Invest Downloaded from infor
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1568 I. J. Radiation Oncology d Bio
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