LIFE01200604005 Shri Somnath Ghosh - Homi Bhabha National ...

CONTENTS
Page No.
SYNOPSIS…………………………………………………………………………. 1-28
LIST OF FIGURES
Fig. 1.1 Pie-chart showing the percentage of radiation exposure…….. 31
Fig. 1.3A Regulation of Cell Cycle by p53 and p21…………………. 50
Fig. 1.3B Pathways of DSB repair……………………………………. 53
Fig. 1.4 Effects of ionizing radiation on cellular response systems….. 62
Fig.1.5. Biological consequences of clustered DNA damage………... 71
Fig. 1.6 Radiation induced bystander effect…………………………. 76
Fig. 3.1.1 Relative radioresistance of three cell line…………………. 96
Fig. 3.1.2 Relative expression of genes in A549 cells……………….. 98
Fig. 3.1.3 Validation of microarray data by RT-PCR………………… 126
Fig.3.1.4 Gene expression of ATM, DNA-PK, Rad52 and MLH1…… 129
Fig. 3.1.5 Repair kinetics as determined by γ-H2AX foci……………… 130
Fig. 3.1.6A Radiation induced foci of DNA damage response proteins... 131
Fig. 3.1.7 Translocation of phospho-p53……………………………….. 133
Fig. 3.1.8 Response of A549 and MCF-7………………………………. 134
Fig.3.1.9 Stabilization of DNA-PK mRNA in A549…………………… 136
Fig.3.1.10 Effect of Rad52 and MLH1…………………………………. 137
Fig.3.2.1 Clonogenic Cell Survival of A549……………………………. 140
Fig.3.2.2 Gene expression of ATM, DNA-PK, ATR, Chk1 and Chk2…. 141
Fig.3.2.3 Immunofluorescence staining and Image analysis…………….. 142
Fig.3.2.4 Gene expression of Bax and Bcl-2…………………………….. 143
Fig.3.3.1.1 Clonogenic Cell Survival of A549 cells……………………... 145
Fig.3.3.1.2 Formation of γ-H2AX foci…………………………………... 147
Fig.3.3.1.3 Phosphorylation of ATM……………………………………. 149
Fig.3.3.1.4 Phosphorylation of ATR…………………………………….. 150
Fig.3.3.1.5 Phosphorylation of BRCA1…………………………………. 151
Fig.3.3.1.6 Phosphorylation of Chk1 and Chk2…………………………. 153
Fig.3.3.1.7a Phosphorylation of ERK…………………………………… 154
Fig.3.3.1.7b Phosphorylation of JNK…………………………………… 155
Fig.3.3.1.8 Apoptosis in carbon irradiated A549 cells…………………... 156
Fig. 3.3.2.1 Clonogenic Cell Survival of A549 cells……………………. 158
Fig. 3.3.2.2 Phosphorylation of H2AX…………………………………. 160
Fig.3.3.2.3 Phosphorylation of ATM…………………………………… 163
Fig.3.3.2.4 Phosphorylation of ATR……………………………………. 164
Fig.3.3.2.5 Phosphorylation of TP53……………………………………. 165
Fig.3.3.2.6 Phosphorylation of Chk2……………………………………. 166
Fig.3.3.2.7 Apoptosis in oxygen irradiated A549 cells………………….. 167
Fig.3.4.1Bystander effect in similar cells (A549 Vs A549)……………... 170
Fig.3.4.2 Bystander effect in dissimilar cells (A549 Vs U937)…………. 171