LIFE01200604005 Shri Somnath Ghosh - Homi Bhabha National ...

More documents

Recommendations

Info

SYNOPSIS Percentage Survival 100 80 60 40 20 0 MCF7 INT407 A549 0Gy 2Gy 5X2Gy 10Gy Radiation Dose Fig. 3.1A. Relative radioresistance of three cell line viz. MCF-7, INT 407 and A 549 Data represents means ± SE of three independent experiments. Lane 1, control unirradiated A549 cell line. Key; Lane 2, 2 Gy acute dose ( single dose that was delivered in each fraction ) of 60 Co γ-irradiation Lane 3 2 Gy of 60 Co γ-irradiation daily over a period of 5 days, Lane 4, 10 Gy acute (which is the sum total of all the fraction ) 60 Co γ-irradiation. Ratio of ATM to Actin 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0.0 Con 2 Gy 5X2 Gy 10 Gy Treatment MCF INT407 A549 Fig. 3.1B. Gene expression of ATM in MCF-7, INT 407 and A 549 cells. Key; Lane 1, control unirradiated A549 cell line. Lane 2, 2 Gy acute dose ( single dose that was delivered in each fraction ) of 60 Co γ-irradiation Lane 3 2 Gy of 60 Co γ-irradiation daily over a period of 5 days, Lane 4, 10 Gy acute (which is the sum total of all the fraction ) 60 Co γ-irradiation. 9
SYNOPSIS Ratio of DNA-PK to Actin 2.5 2.0 1.5 1.0 0.5 0.0 1 2 3 4 Lane Fig. 3.1C. Gene expression of DNA-PK in A 549 cells. Key; Lane 1, control unirradiated A549 cell line. Lane 2, 2 Gy acute dose ( single dose that was delivered in each fraction ) of 60 Co γ-irradiation Lane 3 2 Gy of 60 Co γ-irradiation daily over a period of 5 days, Lane 4, 10 Gy acute (which is the sum total of all the fraction ) 60 Co γ-irradiation. Relative phosphorylation of p53 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 p-TP53 Con 2Gy 5X2Gy 10Gy Loading control (ponceau) Con 2Gy 5X2Gy 10Gy Fig. 3.1D Expression of phospho-p53 in A 549 cells. Key; Lane 1, control unirradiated A549 cell line. Lane 2, 2 Gy acute dose ( single dose that was delivered in each fraction ) of 60 Co γ-irradiation Lane 3 2 Gy of 60 Co γ-irradiation daily over a period of 5 days, Lane 4, 10 Gy acute (which is the sum total of all the fraction ) 60 Co γ-irradiation. 10
Page 1: RADIATION INDUCED SIGNALING IN MAMM
Page 4 and 5: DECLARATION I, hereby declare that
Page 6 and 7: CONTENTS Page No. SYNOPSIS………
Page 8 and 9: 2.11 Measurement of NO production
Page 10 and 11: PREAMBLE SYNOPSIS Ionising radiatio
Page 12 and 13: SYNOPSIS Aims and Objectives: To St
Page 14 and 15: SYNOPSIS 2.6 Immunofluorescence sta
Page 16 and 17: SYNOPSIS ATM gene expression, which
Page 20 and 21: SYNOPSIS Ratio of Rad52 to Actin Ra
Page 22 and 23: Relative Phosphorylation 45 40 35 3
Page 24 and 25: SYNOPSIS Fig. 3.3A Clonogenic Cell
Page 26 and 27: SYNOPSIS (A) Av No. of phospho BRCA
Page 28 and 29: SYNOPSIS Percentage Survival 120 10
Page 30 and 31: SYNOPSIS Fig.3.4B. Existance of cro
Page 32 and 33: SYNOPSIS Fig. 3.4EDNA damage in EL-
Page 34 and 35: SYNOPSIS subsequent cytotoxicity ca
Page 36 and 37: SYNOPSIS [4] Hall, E. J. Radiobiolo
Page 38 and 39: CHAPTER 1 INTRODUCTION INTRODUCTION
Page 40 and 41: CHAPTER 1 INTRODUCTION 15.8% 9.56%
Page 42 and 43: CHAPTER 1 INTRODUCTION far apart an
Page 44 and 45: CHAPTER 1 INTRODUCTION and are more
Page 46 and 47: CHAPTER 1 INTRODUCTION 1.3 DNA dama
Page 48 and 49: CHAPTER 1 INTRODUCTION associates w
Page 50 and 51: CHAPTER 1 INTRODUCTION are unable t
Page 52 and 53: CHAPTER 1 INTRODUCTION Perhaps one
Page 54 and 55: CHAPTER 1 INTRODUCTION occurs at DS
Page 56 and 57: CHAPTER 1 INTRODUCTION related prot
Page 58 and 59: CHAPTER 1 INTRODUCTION damage must
Page 60 and 61: CHAPTER 1 INTRODUCTION At the prese
Page 62 and 63: CHAPTER 1 INTRODUCTION predominant
Page 64 and 65: CHAPTER 1 INTRODUCTION provide a me
Page 66 and 67: CHAPTER 1 INTRODUCTION hypersensiti
Page 68 and 69:
CHAPTER 1 INTRODUCTION cycle-specif
Page 70 and 71:
CHAPTER 1 INTRODUCTION 1.4 Overview
Page 72 and 73:
CHAPTER 1 INTRODUCTION 1.4.1 Radiat
Page 74 and 75:
CHAPTER 1 INTRODUCTION interaction
Page 76 and 77:
CHAPTER 1 INTRODUCTION p90S6 kinase
Page 78 and 79:
CHAPTER 1 INTRODUCTION CD4 (formerl
Page 80 and 81:
CHAPTER 1 INTRODUCTION unrepaired d
Page 82 and 83:
CHAPTER 1 INTRODUCTION well to the
Page 84 and 85:
CHAPTER 1 INTRODUCTION 1.6 Radiatio
Page 86 and 87:
CHAPTER 1 INTRODUCTION becomes pote
Page 88 and 89:
CHAPTER 2 MATERIALS AND METHODS MAT
Page 90 and 91:
CHAPTER 2 MATERIALS AND METHODS 2.2
Page 92 and 93:
CHAPTER 2 MATERIALS AND METHODS res
Page 94 and 95:
CHAPTER 2 MATERIALS AND METHODS Arr
Page 96 and 97:
CHAPTER 2 MATERIALS AND METHODS PBS
Page 98 and 99:
CHAPTER 2 MATERIALS AND METHODS the
Page 100 and 101:
CHAPTER 2 MATERIALS AND METHODS 2.1
Page 102 and 103:
CHAPTER 3 RESULTS RESULTS 93
Page 104 and 105:
CHAPTER 3 RESULTS fractionated regi
Page 106 and 107:
CHAPTER 3 RESULTS On comparison of
Page 108 and 109:
CHAPTER 3 RESULTS Table 3.1.2A List
Page 110 and 111:
CHAPTER 3 RESULTS 80 NM_002185 IL7R
Page 112 and 113:
CHAPTER 3 RESULTS SAA2 149 AU134977
Page 114 and 115:
CHAPTER 3 RESULTS 221 LOC643167 RNA
Page 116 and 117:
CHAPTER 3 RESULTS 299 BF244402 FBXO
Page 118 and 119:
CHAPTER 3 RESULTS CDK4) 57 AU152107
Page 120 and 121:
CHAPTER 3 RESULTS 134 AL045793 METT
Page 122 and 123:
CHAPTER 3 RESULTS 58 AF237813 ABAT
Page 124 and 125:
CHAPTER 3 RESULTS 127 AI809749 ORMD
Page 126 and 127:
CHAPTER 3 RESULTS 31 BE615699 UNC84
Page 128 and 129:
CHAPTER 3 RESULTS 47 AF026303 SULT1
Page 130 and 131:
CHAPTER 3 RESULTS 117 BF062193 CYor
Page 132 and 133:
CHAPTER 3 RESULTS 187 AL036662 ---
Page 134 and 135:
CHAPTER 3 RESULTS 52 AV686514 EMP2
Page 136 and 137:
CHAPTER 3 RESULTS exposed to 10 Gy
Page 138 and 139:
CHAPTER 3 RESULTS Fig.3.1.4 Gene ex
Page 140 and 141:
CHAPTER 3 RESULTS Fig. 3.1.6 Radiat
Page 142 and 143:
CHAPTER 3 RESULTS Fig. 3.1.7 Transl
Page 144 and 145:
CHAPTER 3 RESULTS 3.1.9 Stabilizati
Page 146 and 147:
CHAPTER 3 RESULTS 23±1.5% (Fig. 3.
Page 148 and 149:
CHAPTER 3 RESULTS whereas only the
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
CHAPTER 3 RESULTS with equal doses
Page 156 and 157:
CHAPTER 3 RESULTS Fig.3.3.1.2 Forma
Page 158 and 159:
CHAPTER 3 RESULTS Fig.3.3.1.3 Phosp
Page 160 and 161:
CHAPTER 3 RESULTS (7.5±0.64) (Fig.
Page 162 and 163:
Page 164 and 165:
CHAPTER 3 RESULTS Fig.3.3.1.7b Phos
Page 166 and 167:
CHAPTER 3 RESULTS 3.3.2 Oxygen beam
Page 168 and 169:
CHAPTER 3 RESULTS in all the cells.
Page 170 and 171:
CHAPTER 3 RESULTS 3.3.2.3 Radiation
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
CHAPTER 3 RESULTS Fig.3.3.2.7 Apopt
Page 178 and 179:
CHAPTER 3 RESULTS PMA stimulated U9
Page 180 and 181:
CHAPTER 3 RESULTS Fig.3.4.2. Exista
Page 182 and 183:
CHAPTER 3 RESULTS up-regulation of
Page 184 and 185:
CHAPTER 3 RESULTS Fig.3.4.3.2 NO pr
Page 186 and 187:
CHAPTER 3 RESULTS 3.4.3.4 Apoptotic
Page 188 and 189:
CHAPTER 3 RESULTS 3.4.4 Bystander e
Page 190 and 191:
CHAPTER 3 RESULTS Fig.3.4.4b DNA da
Page 192 and 193:
CHAPTER 3 RESULTS Fig.3.4.5a Gene e
Page 194 and 195:
CHAPTER 4 DISCUSSION DISCUSSION 185
Page 196 and 197:
CHAPTER 4 DISCUSSION directly expos
Page 198 and 199:
CHAPTER 4 DISCUSSION dose is delive
Page 200 and 201:
CHAPTER 4 DISCUSSION A clear cause
Page 202 and 203:
CHAPTER 4 DISCUSSION 4.2 Proton bea
Page 204 and 205:
CHAPTER 4 DISCUSSION Although the e
Page 206 and 207:
CHAPTER 4 DISCUSSION Similar number
Page 208 and 209:
CHAPTER 4 DISCUSSION the mechanism
Page 210 and 211:
CHAPTER 4 DISCUSSION Thus unlike th
Page 212 and 213:
CHAPTER 4 DISCUSSION different from
Page 214 and 215:
CHAPTER 4 DISCUSSION upregulates ma
Page 216 and 217:
CHAPTER 4 DISCUSSION Numerous studi
Page 218 and 219:
CHAPTER 4 DISCUSSION inhibition of
Page 220 and 221:
CHAPTER 4 DISCUSSION The survival o
Page 222 and 223:
CHAPTER 5 REFERENCES [1] Khan, F. T
Page 224 and 225:
CHAPTER 5 REFERENCES [19] Woo, R. A
Page 226 and 227:
CHAPTER 5 REFERENCES [35] Nghiem, P
Page 228 and 229:
CHAPTER 5 REFERENCES [52] McKay, B.
Page 230 and 231:
CHAPTER 5 REFERENCES [69] Cary, R.
Page 232 and 233:
CHAPTER 5 REFERENCES [84] Uziel, T.
Page 234 and 235:
CHAPTER 5 REFERENCES [98] Liu, Y.;
Page 236 and 237:
CHAPTER 5 REFERENCES [111] Fei, P.;
Page 238 and 239:
CHAPTER 5 REFERENCES [127] Frank, K
Page 240 and 241:
CHAPTER 5 REFERENCES [141] Pierce,
Page 242 and 243:
CHAPTER 5 REFERENCES [156] Roots, R
Page 244 and 245:
CHAPTER 5 REFERENCES [171] Xia, Z.;
Page 246 and 247:
CHAPTER 5 REFERENCES (MAP) kinase c
Page 248 and 249:
CHAPTER 5 REFERENCES phosphorylatio
Page 250 and 251:
CHAPTER 5 REFERENCES [211] Anderson
Page 252 and 253:
CHAPTER 5 REFERENCES [229] Konca, K
Page 254 and 255:
CHAPTER 5 REFERENCES [245] Kastan,
Page 256 and 257:
CHAPTER 5 REFERENCES [261] Miralbel
Page 258 and 259:
CHAPTER 5 REFERENCES [278] Weizman,
Page 260 and 261:
CHAPTER 5 REFERENCES [294] Zhou, H.
Page 262 and 263:
PUBLICATIONS AND PRESENTATIONS Publ
Page 264 and 265:
Mutation Research 729 (2012) 61-72
Page 266 and 267:
S. Ghosh, M. Krishna / Mutation Res
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
Page 278 and 279:
12 S. Ghosh et al. / Mutation Resea
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288 and 289:
192 S. Ghosh et al. / Mutation Rese
Page 290 and 291:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Cancer Invest Downloaded from infor
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304 and 305:
1568 I. J. Radiation Oncology d Bio
Page 306 and 307:
Page 308 and 309:
Page 310:
show all

LIFE01200604005 Shri Somnath Ghosh - Homi Bhabha National ...

Create successful ePaper yourself

Delete template?

Save as template?