Tail Flick Meter for Evaluating Thermal Analgesia - Harvard Apparatus

analgesia
Tail Flick Meter for Evaluating Thermal Analgesia
Tail Flick Meter
Key Features
• Optimal detection due to perfect alignment of
heat stimulus and photo beam trigger
• Photo beams with adjustable sensitivity
• A light beam shows the point on which the heat
source will focus
• Manual and remote timer
• Groove for correct tail placement
• Automatic cut-off
• Computer interface
Parameters Measured
• Time latency responses to thermal stimulus
Components Included
• Control Unit with RS-232 port to PC
• Stimulation unit
• Mouse tail adapter
• Holder for rat or mouse
• Footswitch
• SeDaCom software
• Instruction manual
• Calibration certificate
• Cables and connectors
• Set of spare fuses
• 2 year warranty
Options
• LE7000 Thermal printer
• LE7106T Tail-Temperature recorder
Tail Flick Meter
This system features radiant heat applied on the animal’s tail; when the
animal feels discomfort, it reacts by a sudden tail’s movement (tail flick)
which automatically stops the stimulation and the timer for the
measurement of the animal reaction time (period from the beginning of
the stimulation until detection of the animal’s response).
This test has proved particularly sensitive for studying the analgesic
properties of pharmacological substances. It can also be used to evaluate
basal thermal pain sensitivity or to study putative genetic differences
among control animals.
The LE7106 Tail-flick Meter consists of a stimulation unit (containing the
halogen lamp for the heat stimulus) and an electronic control unit. The
system can be used for rats and mice of different sizes. The animals are
placed with its tail protruding within a restraining tube on the platform of the
stimulus unit. The animal’s tail is positioned on a slot of adjustable width
equipped with a groove that guarantees a correct placement. A remote footswitch
controls the test start/stop allowing rapid hands-free experiments.
A photo beam with adjustable sensitivity detects the tail flick and the
latency is automatically presented on a digital display on the control unit.
Measurements of reaction time are given with a 0.1 precision. A cut-off
time can be set to avoid tissue damage (by default: 20 s). The groove
system for the tail and the adjustment of response sensitivity ensure
optimum repeatability and reliability of results.
SeDacom software supplied with the unit can be used to automatically
record the results on a PC through a RS-232 port.
Specifications
Control Unit
Dimensions
Stimulation Unit
Dimensions
Power Supply
Material Composition
Computer Requirements
Maximum Number
of Stations
Certifications
Model Product Order # Price $
LE7106 Tail Flick Analgesia Meter BH1 76-0293 6,493.00
Including SeDaCom Software
Options
350 (W) x 350 (D) x 130 (H)
400 (W) x 140 (D) x 155 (H)
110V/220V, 50/60Hz
Methacrylate, halogen lamp
PC (Windows 95, 98, ME, NT, 2000 and XP)
1 per computer (multiple set-ups
also available under request)
CE compliant
LE7000 Thermal Printer BH1 76-0114 1,341.00
LE7106T Tail-Temperature Recorder BH1 76-0294 1,644.00
Citations
Andriambeloson E et al. (2006) Interleukin-6 attenuates the development of experimental
diabetes-related neuropathy. Neuropathology. 26(1): 32-42. (rat, France)
Cendán CM et al. (2005) Antinociceptive effects of haloperidol and its metabolites in the
formalin test in mice. Psychopharmacol. 182(4): 485-493. (mouse. Spain)
Grillet N et al. (2005) Generation and characterization of Rgs4 mutant mice. Mol. Cell. Biol.
25(10): 4221-4228. (mouse, France)
Moncada A et al. (2005) Inhibitors of serine/threonine protein phosphatases antagonize the
antinociception induced by agonists of alpha 2 adrenoceptors and GABAB but not kappa-opioid
receptors in the tail flick test in mice. Pain 114(1-2): 212-220. (mouse, Spain)
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Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
Panlab | Harvard Apparatus • Spain +34934190709 • international +34834750697 • fax +34934750699 • www.panlab.com

analgesia
Tail Flick Meter and Hot Plate Analgesia Meter
BH1 52-9487 Tail Flick
Analgesia Meter
BH1 52-8570
Hot Plate Analgesia Meter
with Footswitch
• Digital display of plate temperature
• Digital timer with remote start stop
• Accurate temperature control from 35°C to
65°C (±0.3°C)
• For rapid screening of analgesic drugs using rats
(as described by D’Amour and Smith)
Tail Flick Analgesia Meter
This meter measures a rat’s reaction time to radiant energy, from a 150
watt light source. The beam is focused on its tail using a parabolic
reflector. The energy of the light source can be adjusted and the display
indicates, as a percentage, how much energy is being utilized.
An optical sensor is located underneath the focused light source. The rat
should be positioned such that its tail obscures the focused light source
from the sensor. When the system is started, either using the supplied
footswitch or front panel mounted start key the light source illuminates
and a timer starts counting in tenths of a second. When the rat’s tail
flicks, indicating its pain threshold, it uncovers the sensor. This tail
movement turns off the timer and light source. Reaction time can be read
directly from the display in seconds and tenths of a second.
A standard parallel port permits connection to a printer to record the trial
number, energy level and reaction time. A calibration facility allows the light
source to be set to the desired level before commencing with the experiment.
Mouse version is also available. Please contact Harvard Apparatus for details.
Specifications
Lamp Intensity 150 W, adjustable between 0 and 100% in 1%
increments
Timer Range
0 to 99 min 59.9 secs in 0.1 sec steps
Printer Interface Centronix parallel
Lamp Heat Control Digital DC regulated
Dimensions, H x W x D 260 x 450 x 260 mm (10 x 18 x 10 in)
Weight
9 kg (19.8 lbs)
Product Order # Price $
Tail Flick Analgesia Meter, 115 VAC, 60 Hz BH1 52-9487 3,853.00
Tail Flick Analgesia Meter, 240 VAC, 50 Hz BH1 52-9495 3,853.00
Hot Plate Analgesia Meter
The Harvard Apparatus UK Hot Plate Analgesia Meter is a sophisticated
temperature control and timing system, and has been designed to perform
rapid and precise screening of the narcotic type analgesic drugs (Morphine,
Codeine, etc.) according to the Eddy and Leimback hot plate test. This method
evaluates the reaction time of mice when a heat stimulus is applied to the
plantar surface. This reaction time increases when a central analgesic is
administered to the animal. This system can be used with both mice and rats.
Utilizing a simple user interface the user can quickly and easily set up the
required hot plate temperature and a large easy to read LED display shows
the current temperature.
The timer requires a single press of the Start / Stop Key to start and another
press to stop, with reset automatically executed when timing is initiated.
This function is also duplicated by a remote Start/Stop footswitch (supplied).
The reaction time is again clearly displayed on a large LED display.
Using digital electronics, the hot plate temperature is constantly
monitored and regulated to ensure the actual temperature and the desired
temperature accurately match. The system also monitors the heating
characteristics of the system and uses this data to minimize heating
overshoot, providing faster temperature stabilization.
Specifications
Temperature Range 35° to 65°C
Temperature Stability ±0.3°C
Temperature Control Digital proportional PWM
Timer
Digital readout in 0.1 sec increments
Timer Range
0 to 9 mins 59 secs 9 tenths of a sec
Remote
Momentary make to start/stop
Remote Socket
6.35 mm 2 pole jack
Animal Container Two furnished, large round cylinders
Mains Supply Voltage 115 VAC / 230 VAC, 50/60 Hz (factory set)
Dimensions, H x W x D 128 x 275 x 293 mm (5 x 10.8 x 11.5 in)
Weight
4.5 kg (9.9 lb)
Product Order # Price $
Hot Plate Analgesia Meter 110 to 115 VAC, 60 Hz BH1 52-8570 3,234.00
Hot Plate Analgesia Meter 220 to 230 VAC, 50 Hz BH1 52-8588 3,234.00
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
Panlab | Harvard Apparatus • Spain +34934190709 • international +34834750697 • fax +34934750699 • www.panlab.com
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