Tail Flick Analgesia Meter Manual - Harvard Apparatus

Tail Flick Analgesia Meter
Users Manual
Hot Plate Analgesia Meter
Heater
Power
42.5
P
0.00.5
START
STOP
P
Temperature °C
HARVARD
APPARATUS
Time h.mm.ss
HARVARD
A P P A R A T U S

Contents
2
Tail Flick Analgesia Meter Instruction Data
Description
Introduction
General Description
Precautions
Front Panel Layout
Rear Panel Layout
Controls
Switching On
Setting the Temperature
Viewing the Temperature
Operation
Heating
Using the Timer
General Operation
Bibliography
Maintenance
Cleaning
Replacing Mains Fuses
Technical Specification
Support
Page
3
4
6
7
8
8
9
10
10
11
11
12
12
13
14

Introduction
3
General Description
The New Harvard Hot Plate Analgesia Meter is a sophisticated
temperature control and timing system, and has been designed
to perform rapid and precise screening of the narcotic type
analgesic drugs (Morphine, Codeine, etc) according to the Eddy
and Leimback hot plate test. This method evaluates the reaction
time of mice when a heat stimulus is applied to the plantar
surface. This reaction time increases when a central analgesic
is administered to the animal.
Utilising a simple user interface the user can quickly and easily
set up the required hot plate temperature, and a large easy to
read LED display shows the current temperature.
The timer requires a single press of the Start / Stop Key to start
and a further press to stop, with reset automatically executed
when timing is initiated. This function is also duplicated by a
remote Start / Stop footswitch (supplied). The reaction time is
again clearly displayed on a large LED display.
Using digital electronics the hot plate temperature is constantly
monitored and regulated to ensure the actual temperature
accurately matches the desired temperature, and hence no
temperature calibrations will ever be required.
The system is also intelligent and learns the heating
characteristics of the hot plate allowing for fast heating up with
minimal temperature over-shoot and excellent temperature
stabilisation characteristics.

Introduction
4
Precautions
This Equipment has been designed for use with an earthed
mains supply and should not be operated when not earthed.
Connect the power cable of the unit to a suitable three pin
plug in accordance with the following :
Brown lead to the LIVE terminal of your plug
Blue lead to the NEUTRAL terminal of your plug
Green & Yellow lead to the EARTH terminal of your plug
Before operating the Hot Plate ensure that the mains supply
voltage is compatible.
The unit is factory set to 110 Volts or 230 Volts (50/60Hz) as
required.
The mains inlet socket, located on the rear panel, also
incorporates the two mains fuses.
The fuses should only be replaced with those of an identical
type (see the specifications on page 13)
When replacing the mains fuse always ensure the equipment
is disconnected from the mains supply.
Warning : Use of this equipment in a manner not specified
by the manufacturer may impair the protection provided by
this equipment and could invalidate the warranty.
This unit is intended for use as a research or teaching tool
ONLY and is not authorised for use as a component in a life
support device or other clinical system. Therefore neither we
nor our agents can be held responsible should this notice be
disregarded.

Introduction
5
Precautions
Before operating this apparatus care should be taken to situate
the unit in a suitable location, in order to provide optimum
performance. Failure to do so may impair the units operation.
The unit should always be operated on a level, clean and dry
secure surface.
Although the unit only requires minimum free air to circulate
around it, it is recommended that a one inch (2.5 cm) clearance
is provided around the unit where possible.

Introduction
6
Front Panel Layout
1 2 3 4 5 6 7
Hot Plate Analgesia Meter
Heater
Power
42.5
P
0.00.5
START
STOP
P
Temperature °C
HARVARD
APPARATUS
Time h.mm.ss
8 9 10 11
1 Temperature Display
2 Temperature Increase Key
3 Heater Indicator
4 Power Indicator
5 Timer Display
6 Timer Start/Stop Key
7 Unused Programming Key
8 Temperature Set Key
9 Temperature Decrease Key
10 Unused Programming Key
11 Unused Key Programming Key

Introduction
7
Rear Panel Layout
1
2
3
4.
1 Remote Start/Stop Socket
2 Mains Fuse Compartment
3 Mains Inlet Socket
4 Power Switch

Controls
8
Switching On
Connect the unit to a suitable mains supply, and switch on
using the rear panel mounted Mains Switch (see page 7
item 4). The front panel mounted green Power Indicator (see
page 6 item 4) will illuminate and both the red LED Displays
(see page 6 items 1 & 5) will illuminate.
Setting the Temperature
When the the unit is first switched on the Temperature Display
(see page 6 item 1) will indicate the current temperature of
the top plate.
To adjust the desired temperature briefly press the
Temperature Set “P” Key (see page 6 item 8), at this point
the display will alternately show SP1 and the current set
temperature.
SP1 P
>>>
37.0 P
OUT 1 OUT 2
OUT 1 OUT 2
To change the set temperature use the Temperature Increase
and Decrease Keys (see page 6 items 2 & 9).
When the desired temperature has been entered briefly press
the Temperature Set “P” Key (see page 6 item 8) again to
store and return back to its default view of current temperature.
Note: If the Temperature Set P Key (see page item 8) is
continually held the system will enter the programming mode
which is user locked. Please try to avoid entering this menu,
if you do so switch of the mains power and wait for 10seconds
before switching on again.

Controls
9
Viewing the Set Temperature
To view the set temperature briefly press the Temperature
Set “P” Key (see page 6 item 8), at this point the display will
alternately show SP1 and the current set temperature.
SP1 P
>>>
37.0 P
OUT 1 OUT 2
OUT 1 OUT 2
Press the Temperature Set “P” Key (see page 6 item 8)
again or wait a few seconds for the display to return to the
default view; indicating the current temperature.
Note: If the Temperature Set P Key (see page item 8) is
continually held the system will enter the programming mode
which is user locked. Please try to avoid entering this menu,
if you do so switch of the mains power and wait for 10seconds
before switching on again.

Operation
10
Heating
If the Set Temperature is of a value greater than that of the
current plate temperature then the unit will automatically
switch on the heater, confirmed by the red front panel mounted
Heater Indicator (see page 6 item 3). As the unit nears the
set temperature the heater will be used in its proportional
output mode, this effectively pulses the heater so as to reduce
the heat output, and consequently minimises the likely hood
of a large temperature over-shoot when heating up. Once
the desired temperature has been reached the heater will be
switched off.
As the temperature falls below the desired value so the heater
will be proportional switched on again as required.
Using the Timer
To start the timer briefly press the timer Start/Stop Key (see
page 6 item 6) the display will start counting in tenths of a
second.
To stop the timer briefly press the timer Start/Stop Key (see
page 6 item 6). The timer will stop and display the elapsed
time in the form Mins/Seconds/Tenths of a Second.
Further presses of the Start/Stop Key (see page 6 item 6)will
in turn Start and Stop the timer, each time the timer is started
it will automatically reset to zero.
The function of the Start/Stop Key can be replicated by the
supplied footswitch when connect to the rear panel mounted
Remote Socket (see page 7 item 1), although any kind of
non-latching switch can be used (see the specifications on
page 13).
Note: The timer module includes several specific programming
keys (see page 6 items 7, 10 & 11) that are not, at present
utilised. These keys should not be used unless instructed to
do so by a Harvard Apparatus Engineer.
If you accidentally change any settings switch off the power.

Operation
11
Using the Timer
Switch the unit on again then briefly press the P key (see
page 6 item 10) the display should show 9.99.9 if this is not
so, use the up and down keys (see page 6 items 7&10) to
achieve this.
Now wait a few seconds for the display to return to its default
status.
If whilst using the timer you obtain unpredictable results
contact your nearest Harvard Apparatus agent.
General Operation
With the unit connected and switched on, as described
previously on Page 8 set the desired temperature, using the
routine described on Page 8. Wait for the temperature to
reach the desired value and allow to stabilise for 15 minutes.
Drop the animal to be tested onto the the hotplate and start
the timer using the footswitch or front panel mounted stop /
start key.
Stop the timer by depressing the footswitch or front panel
mounted start / stop key once again, as soon as the animal
is seen to lick its paws
Remove the animal from the hotplate. The counter will show
the reaction time of the animal and this should be recorded.
Bibliography
Nathan B. Eddy & Doroth Leimbach: "Synthetic Analgesics.
II Dithienylbutenyl and dithienylbutylamines " Journal of
Pharmacological Experimental Therapeutics. 107 pages 385-
393 1953.

Maintenance 12
Cleaning
!
WARNING
Always ensure the unit is completely isolated from the mains
supply whilst cleaning.
No special maintenance is required other than periodic cleaning
using a soft cloth and a mild detergent on the top plate.
Use a damp cloth to clean the front and rear panels, a mild
detergent should be carefully used for heavily marked areas.
Replacing the Mains Fuses
If the unit fails to power up it is recommended that the mains
fuses be checked before returning the unit for repair or service.
Mains fuse are of the quick blow 20mm type.
If your unit is rated for 110 - 115 Volt use then the mains fuses
must be 10 Amps.
If your unit is rated for 220 - 230 Volt use then the mains fuses
must be 5 Amps.
To change the mains fuses, remove the mains inlet cable from
the rear panel mounted mains inlet socket (see page 7 item 3),
and using a small flat bladed screw driver hook out the small
enclosed fuse compartment (see page 7 item 2).
Remove the two fuses and check both of them, if in doubt replace
both fuses.
When complete close the fuse compartment securely, reconnect
to the mains supply and switch on.
If the unit persistently blows the mains fuse, the unit should be
returned to your Harvard Apparatus agent for service.

Technical
13
Technical Specification
Temperature Range
Temperature Stability
Timer Range
Remote
Remote Socket
Mains Supply Voltage
Mains Fuse
Dimensions
Weight
35°C to 65°C
+/- 0.3°C
0 to 9Minutes 59 Seconds 9 Tenths of a Second
Momentary make to Start / Stop
6.35mm 2Pole Jack
115 Volts / 230 Volts AC 50/60Hz (Factory Set)
5 Amp 20mm Quick Blow (220 - 230 Volt Units)
10 Amp 20mm Quick Blow (110 - 115 Volt Units)
275mm x 128mm x293mm (W x H x D)
4.5Kg
HARVARD APPARATUS 52-8588 MANUAL REVISION 1.02

Support
14
UK, USA, Canada and France Customer Contact Addresses
UK
Harvard Apparatus Ltd
Fircroft Way
Edenbridge
Kent TN8 6HE
Tel +44 (0) 1732 864001
Fax +44 (0) 1732 863356
email sales@harvardapparatus.co.uk
USA
Harvard Apparatus Inc
84 October Road
New Englander Ind Park
Holliston MA 01746
Tel (508) 893 8999
Toll-Free (800) 272 2775
Fax (508) 429 5732
email bioscience@harvardapparatus.com
Canada
Harvard Apparatus Canada
6010 Vanden Abeele Street
St. Laurent
Quebec H4S 1R9
Tel (514) 335 0792
Toll Free (800) 361 1905
Fax (514) 335 3482
email harvardcanada@compuserve.com
France
Harvard Apparatus Sarl
6 Avenue des Andes
Miniparc - bat. 8
91952 Les Ulis Cedex
Tel (1) 64 46 00 85
Fax (1) 64 46 94 38
email harvard@isp.fr
Other Customers
In the event of a breakdown, or if you need assistance, please contact
your local agent. If one is not available contact Harvard Apparatus
directly.

Support
15
For service, technical support or in the event of a system
failure, please contact your local Harvard Apparatus agent,
or our UK office directly.
To ensure we can deal with your enquiry quickly please state
the catalogue number, serial number and date of purchase
in all correspondence. If you are experiencing a fault, also
include a description of the nature of it.
Harvard Apparatus Ltd
Fircroft Way, Edenbridge, Kent, TN8 6HE, United Kingdom
Tel: +44 (0) 1732 864001 Fax: +44 (0) 1732 863356 E-Mail: sales@harvardapparatus.co.uk