Final Exam
Final Exam
Final Exam
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distinguish these possibilities? How would reverse genetics or siRNAs interference help you<br />
with this project? What other experimental approaches may help?<br />
What are the possible functions of Green1, Green3, Green5, and Green6? Recall your double<br />
mutant analysis and pathway predictions and re-interpret in the light of your cloning data. Given<br />
your new-found knowledge, how would you engineer salt-resistant algae that grow fast (i.e. do<br />
not mate), yet flocculate in response to an environmental signal?<br />
Hypothetical Signal Transduction Pathway<br />
signal<br />
?<br />
LR<br />
? ?<br />
7TM GPCR<br />
Galpha/GTP<br />
?<br />
Gbeta/Ggamma<br />
MAPKK<br />
MAPK<br />
TF<br />
Zygote formation (large non-flagellated cells)<br />
? TF TF<br />
TF<br />
TF<br />
?<br />
Flocculation (cells flock together)<br />
Zygote generates four haploid flagellated offspring
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