GC Chromatograms
GC Chromatograms
GC Chromatograms
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<strong>GC</strong> CHROMATOGRAMS | FOODS, FLAVORS, & FRAGRANCES<br />
Fatty Acids<br />
Fatty Acids (Free) on Rtx®-200<br />
Fatty Acids (Free) on Stabilwax®-DA<br />
(30 m, 0.25 mm ID, 0.25 μm)<br />
Peaks<br />
1. Acetic acid<br />
2. Propionic acid<br />
3. Isobutyric acid<br />
4. n-Butyric acid<br />
5. Isovaleric acid<br />
6. n-Valeric acid<br />
7. Isocaproic acid<br />
8. Caproic acid<br />
9. Heptanoic acid<br />
No derivatization<br />
needed!<br />
Peaks<br />
1. Acetic acid<br />
2. Propionic acid<br />
3. Isobutyric acid<br />
4. n-Butyric acid<br />
5. Isovaleric acid<br />
6. n-Valeric acid<br />
7. Isocaproic acid<br />
8. Caproic acid<br />
9. Heptanoic acid<br />
No derivatization<br />
needed!<br />
<strong>GC</strong>_CH00282<br />
Column Rtx®-200, 30 m, 0.25 mm ID, 0.25 μm (cat.# 15023)<br />
Sample<br />
Free fatty acid standard<br />
Conc.:<br />
~10-20 ng/μL<br />
Injection<br />
Inj. Vol.:<br />
0.8 μL split<br />
Inj. Temp.: 250 °C<br />
Split Vent<br />
Flow Rate: 40 mL/min<br />
Oven<br />
Oven Temp: 90 °C<br />
Carrier Gas H2, constant flow<br />
Flow Rate: 1.4 mL/min<br />
Linear Velocity: 40 cm/sec<br />
Detector FID @ 250 °C<br />
Notes<br />
FID sensitivity: 4 x 10 -11 AFS<br />
<strong>GC</strong>_CH00280<br />
Column<br />
Stabilwax®-DA, 30 m, 0.25 mm ID, 0.25 μm<br />
(cat.# 11023)<br />
Sample<br />
Free acid standard<br />
Conc.:<br />
~10-20 ng/μL<br />
Injection<br />
Inj. Vol.: 1.0 μL split (split ratio 50:1)<br />
Inj. Temp.: 250 °C<br />
Oven<br />
Oven Temp: 145 °C<br />
Carrier Gas H2, constant pressure<br />
Linear Velocity: 40 cm/sec<br />
Detector FID @ 250 °C<br />
Notes<br />
FID sensitivity: 2 x 10 -11 AFS<br />
Fatty Acids (Free) on Stabilwax®-DA (30 m, 0.32 mm ID, 0.25 μm)<br />
1<br />
2<br />
3<br />
4<br />
5<br />
6<br />
7<br />
8<br />
9<br />
Great for low to<br />
high molecular<br />
weight fatty acids.<br />
10<br />
11<br />
12<br />
Peaks<br />
1. C4:0 Butyric acid<br />
2. C6:0 Caproic acid<br />
3. C8:0 Caprylic acid<br />
4. C10:0 Capric acid<br />
5. C12:0 Lauric acid<br />
6. C14:0 Myristic acid<br />
7. C16:0 Palmitic acid<br />
8. C18:0 Stearic acid<br />
9. C18:1 Oleic acid<br />
10. C18:2 Linoleic acid<br />
11. C18:3 Linolenic acid<br />
12. C20:0 Arachidic acid<br />
13. C22:0 Behenic acid<br />
Column<br />
Stabilwax®-DA, 30 m, 0.32 mm ID, 0.25 μm<br />
(cat.# 11024)<br />
Sample<br />
Free fatty acid mix<br />
Diluent:<br />
Methanol<br />
Conc.:<br />
100 ppm<br />
Injection<br />
Inj. Vol.:<br />
1 μL splitless (hold 0.25 min)<br />
Liner:<br />
Laminar cup splitter<br />
Inj. Temp.: 250 °C<br />
Purge Flow: 75 mL/min<br />
Oven<br />
Oven Temp: 40 °C to 250 °C at 10 °C/min (hold 15 min)<br />
Carrier Gas H2, constant flow<br />
Flow Rate: 6 mL/min<br />
Detector FID @ 250 °C<br />
13<br />
<strong>GC</strong>_FF00653<br />
1-800-356-1688 or 1-814-353-1300 www.restek.com 641
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