GC Chromatograms

GC CHROMATOGRAMS | FOODS, FLAVORS, & FRAGRANCES
Cholesterols
Underivatized Cholesterol on Rxi®-5ms
A. Temperature program conditions.
Oven temp.: 200 °C (hold 1 min) to 330 °C @ 20 °C/min (hold 7.5 min)
Rxi® Technology!
Exceptionally inert, ultra low-bleed
capillary columns.
Peaks
1. 5--Cholestane (IS)
2. Cholesterol
B. Isothermal conditions will maximize sample throughput.
Oven temp.: 300 °C (hold 10 min)
One column, many
cholesterol methods.
GC_FF00881
Column
Rxi® -5ms, 15 m, 0.25 mm ID, 0.25 μm
(cat.# 13420)
Sample
1,000 μg/mL cholesterol in DMF
1,000 μg/mL 5--cholestane in hexane
25 ng cholesterol, 150 ng 5--cholestane
on column
Injection
Inj. Vol.: 1 μL split (split ratio 20:1)
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)
Inj. Temp.: 250 °C
Carrier Gas He, constant pressure (9.7 psi, 66.9 kPa)
Linear Velocity: 24 cm/sec @ 200 °C
Detector FID @ 340 °C
GC_FF00882
Derivatized Cholesterol on Rxi®-5ms
A. Temperature program conditions.
Oven temp.: 200 °C (hold 1 min) to 330 °C @ 20 °C/min (hold 7.5 min)
Rxi® Technology!
Exceptionally inert, ultra low-bleed
capillary columns.
Peaks
1. 5--Cholestane (IS)
2. Cholesterol
B. Isothermal conditions will maximize sample throughput.
Oven temp.: 300 °C (hold 10 min)
GC_FF00883 Column Rxi®-5ms, 15 m, 0.25 mm ID, 0.25 μm
(cat.# 13420)
Sample
1,000 μg/mL cholesterol in hexane
1,000 μg/mL 5--cholestane in hexane
50 ng derivatized cholesterol, 150 ng
5--cholestane on column
Injection
Inj. Vol.: 1 μL split (split ratio 20:1)
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)
Inj. Temp.: 250 °C
Carrier Gas He, constant pressure (9.7 psi, 66.9 kPa)
Linear Velocity: 24 cm/sec @ 200 °C
Detector FID @ 340 °C
GC_FF00884
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