GC Chromatograms
GC Chromatograms
GC Chromatograms
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<strong>GC</strong> CHROMATOGRAMS | FOODS, FLAVORS, & FRAGRANCES<br />
Cholesterols<br />
Underivatized Cholesterol on Rxi®-5ms<br />
A. Temperature program conditions.<br />
Oven temp.: 200 °C (hold 1 min) to 330 °C @ 20 °C/min (hold 7.5 min)<br />
Rxi® Technology!<br />
Exceptionally inert, ultra low-bleed<br />
capillary columns.<br />
Peaks<br />
1. 5--Cholestane (IS)<br />
2. Cholesterol<br />
B. Isothermal conditions will maximize sample throughput.<br />
Oven temp.: 300 °C (hold 10 min)<br />
One column, many<br />
cholesterol methods.<br />
<strong>GC</strong>_FF00881<br />
Column<br />
Rxi® -5ms, 15 m, 0.25 mm ID, 0.25 μm<br />
(cat.# 13420)<br />
Sample<br />
1,000 μg/mL cholesterol in DMF<br />
1,000 μg/mL 5--cholestane in hexane<br />
25 ng cholesterol, 150 ng 5--cholestane<br />
on column<br />
Injection<br />
Inj. Vol.: 1 μL split (split ratio 20:1)<br />
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)<br />
Inj. Temp.: 250 °C<br />
Carrier Gas He, constant pressure (9.7 psi, 66.9 kPa)<br />
Linear Velocity: 24 cm/sec @ 200 °C<br />
Detector FID @ 340 °C<br />
<strong>GC</strong>_FF00882<br />
Derivatized Cholesterol on Rxi®-5ms<br />
A. Temperature program conditions.<br />
Oven temp.: 200 °C (hold 1 min) to 330 °C @ 20 °C/min (hold 7.5 min)<br />
Rxi® Technology!<br />
Exceptionally inert, ultra low-bleed<br />
capillary columns.<br />
Peaks<br />
1. 5--Cholestane (IS)<br />
2. Cholesterol<br />
B. Isothermal conditions will maximize sample throughput.<br />
Oven temp.: 300 °C (hold 10 min)<br />
<strong>GC</strong>_FF00883 Column Rxi®-5ms, 15 m, 0.25 mm ID, 0.25 μm<br />
(cat.# 13420)<br />
Sample<br />
1,000 μg/mL cholesterol in hexane<br />
1,000 μg/mL 5--cholestane in hexane<br />
50 ng derivatized cholesterol, 150 ng<br />
5--cholestane on column<br />
Injection<br />
Inj. Vol.: 1 μL split (split ratio 20:1)<br />
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)<br />
Inj. Temp.: 250 °C<br />
Carrier Gas He, constant pressure (9.7 psi, 66.9 kPa)<br />
Linear Velocity: 24 cm/sec @ 200 °C<br />
Detector FID @ 340 °C<br />
<strong>GC</strong>_FF00884<br />
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