GC Chromatograms

GC CHROMATOGRAMS | FOOD SAFETY
Food Contaminants
Acrylamide (Reference Standard) on Stabilwax®
Acrylamide (Potato Chip Extract) on Stabilwax®
Peaks
1. Acrylamide
Peaks
1. Acrylamide
1
GC_FF00642
0.5 1.0 1.5 2.0
blank
2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0
Time (min)
GC_FF00643
Column Stabilwax®, 15 m, 0.53 mm ID, 0.50 μm (cat.# 10637)
Sample
Acrylamide standard
Diluent:
Water
Conc.:
25 μg/mL
Injection
Inj. Vol.:
1 μL splitless (hold 0.5 min)
Liner: Auto SYS splitless w/wool (2 mm ID) (cat.# 20830)
Inj. Temp.: 260 °C
Oven
Oven Temp: 100 °C (hold 0.5 min) to 200 °C at 15 °C/min
Carrier Gas He, constant pressure
Linear Velocity: 62 cm/sec @ 100 °C
Detector FID @ 260 °C
Column Stabilwax®, 15 m, 0.53 mm ID, 0.50 μm (cat.# 10637)
Sample
Conc.:
1 g of potato chips spiked with 100 μg acrylamide
Injection
Inj. Vol.:
1 μL splitless (hold 0.5 min)
Liner: Auto SYS splitless w/wool (2 mm ID) (cat.# 20830)
Inj. Temp.: 260 °C
Oven
Oven Temp: 100 °C (hold 0.5 min) to 200 °C at 15 °C/min
Carrier Gas He, constant pressure
Linear Velocity: 62 cm/sec @ 100 °C
Detector FID @ 260 °C
Hexachlorocyclohexanes in Sage QuEChERS Extract (Zoom of GCxGC-TOFMS Contour Plot)
NEW!
GCxGC with orthogonal
Rxi®-5Sil MS and Rtx®-200
columns is a powerful way
to handle complex samples
like dietary supplement
extracts. Note separation
of gamma-HCH from the
isobaric interference above.
Column Rxi®-5Sil MS 30 m, 0.25 mm ID, 0.25 μm (cat.# 13623)
Rtx®-200 1.5 m, 0.18 mm ID, 0.20 μm (cat.# 45001)
Sample
Diluent:
Toluene
Injection
Inj. Vol.:
1 μL splitless (hold 1 min)
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)
Inj. Temp.: 250 °C
Purge Flow:
40 mL/min
Oven
Oven Temp:
Rxi®-5Sil MS: 80 °C (hold 1 min) to 310 °C at 4 °C/min (hold 1.5 min)
Rtx®-200: 90 °C (hold 1 min) to 320 °C at 4 °C/min (hold 1.5 min)
Carrier Gas
He, constant flow
Flow Rate:
Modulation
Modulator Temp.
Offset: 25 °C
Second Dimension
Separation Time:
1.8 mL/min
4 sec
GC_FF1194
Hot Pulse Time: 1.2 sec
Cool Time
between Stages: 0.8 sec
Detector
TOFMS
Transfer Line Temp.: 290 °C
Analyzer Type: TOF
Source Temp.: 225 °C
Electron Energy: 70 eV
Mass Defect: -20 mu/100 u
Solvent Delay Time: 4 min
Ionization Mode: EI
Acquisition Range: 45 to 550 amu
Spectral Acquisition
Rate:
100 spectra/sec
Instrument
LECO Pegasus 4D GCxGC-TOFMS
Notes
See application note GNAN1338 for extraction and cleanup details. A 1.5 m
length of the Rtx®-200 column was trimmed from a 10 m column. Columns
were connected with a universal Press-Tight® connector (cat.# 20429).
628 www.restek.com 1-800-356-1688 or 1-814-353-1300