GC Chromatograms
GC Chromatograms
GC Chromatograms
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<strong>GC</strong> CHROMATOGRAMS | CLINICAL<br />
Amphetamines, Barbiturates<br />
Derivatized Amphetamines (500 ng/mL) on Rxi®-5Sil MS<br />
1<br />
6.0<br />
C<br />
2<br />
Rxi® Technology!<br />
Exceptionally inert, ultra low-bleed<br />
capillary columns.<br />
Excellent peak shape with<br />
short run time.<br />
3 4<br />
7.0 8.0 9.0 10.0 Time (min)<br />
5<br />
<strong>GC</strong>_CF1144<br />
Peaks<br />
1. Amphetamine<br />
2. Methamphetamine<br />
3. MDA<br />
4. MDMA<br />
5. MDEA<br />
c = contaminant<br />
Column<br />
Rxi®-5Sil MS, 30 m, 0.25 mm ID, 0.25 μm<br />
(cat.# 13623)<br />
Sample<br />
Diluent:<br />
Butyl chloride<br />
Conc.:<br />
500 ng/mL HFAA derivatives<br />
Injection<br />
Inj. Vol.:<br />
1 μL splitless (hold 1 min)<br />
Liner:<br />
3.5 mm splitless taper w/wool<br />
(cat.# 22286-200.1)<br />
Inj. Temp.: 250 °C<br />
Purge Flow: 28 mL/min<br />
Oven<br />
Oven Temp: 75 °C to 300 °C at 15 °C/min<br />
Carrier Gas He, constant linear velocity<br />
Linear Velocity: 45 cm/sec, 13.5 psi, 93.1 kPa @ 75 °C<br />
Detector<br />
MS<br />
Mode:<br />
Scan<br />
Transfer Line<br />
Temp.: 250 °C<br />
Analyzer Type: Quadrupole<br />
Source Temp.: 200 °C<br />
Electron Energy: 70 eV<br />
Solvent Delay<br />
Time:<br />
4 min<br />
Tune Type: PFTBA<br />
Ionization Mode: EI<br />
Scan Range: 40-300 amu<br />
Scan Rate: 5 scans/sec<br />
Instrument Shimadzu 2010 <strong>GC</strong> & QP2010+ MS<br />
Underivatized Barbiturates (Acidic/Neutral Drugs) on Rtx®-35<br />
Separation optimized<br />
using Pro ez<strong>GC</strong>!<br />
Peaks<br />
1. Barbital<br />
2. Aprobarbital<br />
3. Butalbital<br />
4. Amobarbital<br />
5. Talbutal<br />
6. Pentobarbital<br />
7. Methohexital<br />
8. Secobarbital<br />
9. Thiopental<br />
10. Hexobarbital<br />
11. Mephobarbital<br />
12. Phenobarbital<br />
Column Rtx®-35, 30 m, 0.32 mm ID, 0.50 μm (cat.# 10439)<br />
Sample<br />
Barbiturates<br />
Injection<br />
Inj. Vol.: 1.0 μL split (split ratio 30:1)<br />
Inj. Temp.: 300 °C<br />
Oven<br />
Oven Temp: 210 °C (hold 2 min) to 300 °C at 7 °C/min (hold 2 min)<br />
Carrier Gas He, constant pressure<br />
Linear Velocity: 35 cm/sec @ 210 °C<br />
Detector FID @ 300 °C<br />
Notes<br />
FID sensitivity: 5.12 x 10 -10 AFS<br />
<strong>GC</strong>_PH00267<br />
www.restek.com 573
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