GC Chromatograms
GC Chromatograms
GC Chromatograms
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<strong>GC</strong> CHROMATOGRAMS | CLINICAL<br />
Cold Medications, Opiates<br />
Underivatized Cold Medications (Basic Drugs) on Rtx®-35 Amine<br />
2<br />
3<br />
4<br />
5<br />
6<br />
7<br />
8<br />
9<br />
Peaks<br />
1. Guaifenesin<br />
2. Pheniramine<br />
3. Phenyltoloxamine<br />
4. Chlorpheniramine<br />
5. Brompheniramine<br />
6. Dextromethorphan<br />
7. Pyrilamine<br />
8. Triprolidine<br />
9. Codeine<br />
10. Hydrocodone<br />
Column Rtx®-35 Amine, 30 m, 0.25 mm ID, 0.50 μm (cat.# 11338)<br />
Sample<br />
Conc.:<br />
1,000 ng/μL<br />
Injection<br />
Inj. Vol.:<br />
1.0 μL split<br />
Inj. Temp.: 250 °C<br />
Oven<br />
Oven Temp: 250 °C to 300 °C at 7 °C/min (hold 7 min)<br />
Carrier Gas He, constant flow<br />
Linear Velocity: 30 cm/sec<br />
Detector<br />
MS<br />
Mode:<br />
Scan<br />
Ionization Mode: EI<br />
Scan Range: 40-450 amu<br />
10<br />
1<br />
3.0<br />
4.0 5.0 6.0<br />
7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0<br />
Time (min)<br />
<strong>GC</strong>_PH00575<br />
Extracted Opiates from Blood on Rxi®-5Sil MS<br />
Rxi® Technology!<br />
Exceptionally inert, ultra low-bleed<br />
capillary columns.<br />
<strong>GC</strong>_CF1150<br />
Peaks<br />
1. Hydrocodone<br />
2. Codeine<br />
3. Oxycodone<br />
4. Hydromorphone<br />
5. 6-Monoacetylmorphine<br />
6. Morphine<br />
* Matrix<br />
Column Rxi®-5Sil MS, 30 m, 0.25 mm ID, 0.25 μm (cat.# 13623)<br />
Sample<br />
Diluent:<br />
Ethyl acetate<br />
Conc.:<br />
100 ng/mL propionic anhydride derivatives<br />
Injection<br />
Inj. Vol.:<br />
1 μL splitless (hold 1 min)<br />
Liner: Splitless taper (4 mm) w/wool (cat.# 22405)<br />
Inj. Temp.: 250 °C<br />
Purge Flow: 100 mL/min<br />
Oven<br />
Oven Temp: 65 °C (hold 1 min) to 315 °C at 15 °C/min<br />
Carrier Gas<br />
He, constant flow<br />
Flow Rate:<br />
1 mL/min<br />
Linear Velocity: 35 cm/sec @ 65 °C<br />
Detector<br />
MS<br />
Mode:<br />
SIM<br />
Transfer Line<br />
Temp.: 250 °C<br />
Analyzer Type: Quadrupole<br />
Solvent Delay<br />
Time:<br />
7 min<br />
Tune Type:<br />
PFTBA<br />
Ionization Mode: EI<br />
Notes<br />
Opiates were spiked into a blood sample and extracted<br />
by SPE, then derivatized with propionic anhydride.<br />
Acknowledgement Data courtesy of Miami Dade County Medical<br />
Examiner Department<br />
www.restek.com 579
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