1985 - Mycological Society of America
1985 - Mycological Society of America
1985 - Mycological Society of America
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character with morphological or anatomical<br />
characters. A reevaluation <strong>of</strong> the list <strong>of</strong> brownrot<br />
agaric qenera resu1.ted in the removal <strong>of</strong><br />
Coprinus as suspect, and the exclusion <strong>of</strong> Lentinus<br />
sensu stricto and Paxillus sensu stricto. Two<br />
new genera, Neolentinus and Heliocybe, are proposed<br />
for :,rown-rot species formerly treated in Lentinus,<br />
and a third new genus, Ussicaulis, for one<br />
previously considered to be a Pleurotus. The<br />
brown-rot genus Tapinella is resurrected as a<br />
link between the hrown-rot family Coni~phoraceae<br />
and the mycorrhizal genus Paxillus, both in<br />
the order Roletales. Recognition <strong>of</strong> the white-rot<br />
genus Lentinula for the Shiitake and allies is<br />
sl~pported I))( decay studies. Six genera <strong>of</strong><br />
agaricoid hyrnenomycetes in at least two orders<br />
now arc consider~d to be brown-rot taxa. Among<br />
agaric genera the formation <strong>of</strong> a brown-rot is a<br />
qerler I c rea t :re.<br />
DON R. REYNOLDS. Natural History Museum,<br />
n o Exposition Boulevard, Los Angeles,<br />
California 90007.<br />
A phylogenetic view <strong>of</strong> the Capnodiaceae<br />
sensu lato.<br />
The Capnodiaceae sensu lato is analyzed<br />
with cladistic technique. The hypothesis<br />
being tested is that the sexual states<br />
and the asexual states <strong>of</strong> monophyletic<br />
pleoholomorphs will have similar trees.<br />
ROBERT H. RIEHL* AND DAVID 0. TOFT. Department <strong>of</strong><br />
Cell Biology, Mayo Clinic, Rochester, MN, 55950. The<br />
Antheridiol Receptor <strong>of</strong> Achlya ambisexualis E87.<br />
We have detected a high affinity bindinz protein<br />
in the cytosol <strong>of</strong> A. ambisexualis male cells ;hat is<br />
specific for antheridiol and a tritium-labeled<br />
analog. Studies have revealed that, in the presence<br />
<strong>of</strong> sodium molybdate, this macromolecule has a<br />
sedimentat ion coefficient <strong>of</strong> 8.3s in sucrose<br />
gradieqts <strong>of</strong> low ionic strength, a Stokes radius <strong>of</strong><br />
56.6 A (Sephacryl 5-300 columns), a molecular<br />
weight <strong>of</strong> approximately 192,000, a frictional ratio<br />
<strong>of</strong> 1.5, and an axial ratio <strong>of</strong> 8.9. The binding<br />
protein can be eluted with 0.24 M KC1 as a single<br />
peak from DEAE-Sephadex A-25 columns. When sucrose<br />
gradients are prepared containing 1 M KC1 without<br />
molybdate, a shift in sedimentation c.oefficient from<br />
8.3s to 3.6s occurs. Recently, we have produced a<br />
monoclonal antibody to one component <strong>of</strong> ,the<br />
antheridiol receptor that cross-reacts with a similar<br />
component <strong>of</strong> the steroid receptors <strong>of</strong> birds and<br />
mammals. These results indicate that this steroid<br />
binding protein from Achlya has in vitro biochemical<br />
properties that are similar to those <strong>of</strong> the steroid<br />
receptors in other organisms and that these<br />
properties may have been evolutionarily conserved.<br />
ROBERT W. ROBERSON, E. S. LUTTRELL, Department <strong>of</strong><br />
Plant Pathology, University <strong>of</strong> Georgia, Athens, GA<br />
30602, and NORMA L. CASHION, formerly CIMMYT. Londres<br />
40, Apdo. Postal 6-641, 06600 Mexico. D.F. Formation<br />
<strong>of</strong> exogenous terminal teliospores in Neovossia indica<br />
(Tilletiaceae).<br />
Teliospores <strong>of</strong> the karnal bunt fungus, Neovossia<br />
indica, develop in extensive shallow cavities<br />
resulting from dissolution <strong>of</strong> the middle layers <strong>of</strong><br />
the pericarp <strong>of</strong> the wheat grain. The cavity is<br />
bounded on the outside by the epidermal and hypodermal<br />
layers <strong>of</strong> the pericarp and on the inside by<br />
the layer <strong>of</strong> cross cells and tube cells. The intercellular<br />
hyphae <strong>of</strong> the fungus line the cavity with a<br />
hymenium-like stroma <strong>of</strong> compacted cells. Short hyphal<br />
37<br />
branches arising from generative cells ir~ the stromal<br />
layer project into the cavity. The tips <strong>of</strong> these<br />
sporogenous branches swell into pyriform teliospore<br />
initials. The primary wall <strong>of</strong> the spore is continuous<br />
with the wall <strong>of</strong> the sporogenous hypha. As the protoplasm<br />
<strong>of</strong> the sporogenous hyphae moves toward its tip<br />
a series <strong>of</strong> empty cells is cut <strong>of</strong>f by septa, and a<br />
final septum delimits the binucleate spore initial.<br />
The two nuclei Fuse and the initial expands enormously<br />
into the globose spiny teliospore. This type<br />
<strong>of</strong> development contrasts with the endogenous formation<br />
<strong>of</strong> teliospores in intercalary hyphal cells with<br />
swollen gelatinous walls reported in Ustilaginaceae..<br />
R.G. ROBERTS. Field and Horticultural Crops Research<br />
Unit, USDA:ARS, R. B. Russell Agricultural Research<br />
Center, P. 0. Box 5677, Athens, GA 30613.<br />
The anamorph <strong>of</strong> Microascus intermedius Emmons & Dodge<br />
During myc<strong>of</strong>loral studies <strong>of</strong> sunflower seeds, an<br />
ascomycete morphologically indistinguishable from<br />
Microascus intermedius was frequently isolated from<br />
low quality seeds. Von Arx erected the genus<br />
Pithoascus for g. nidicola, g. intermedius and other<br />
species lacking anamorphs, with no ascospore germ<br />
pores and with papillate ascomata. The sunflower<br />
isolates <strong>of</strong> M. intermedius were consistently associated<br />
with an undescribed Scopulariopsis anamorph,<br />
especially when grown on sunflower seeds. Conidia<br />
were globose to subglobose, hyaline and measured<br />
4-6(8) X 4-5.5(7.5) micrometers. Annelides measured<br />
4-13 X 2-2.5 micrometers. Fungus-free sunflower<br />
seeds inoculated with ascospores <strong>of</strong> g. intermedius<br />
consistently produced both the anamorphic and teleomorphic<br />
states. Conidial isolates consistently produced<br />
cultures with fertile ascomata <strong>of</strong> g.<br />
intermedius. Both papillate and rostrate ascomata <strong>of</strong><br />
- M. intermedius and g. intermedius were observed in<br />
culture. The identity <strong>of</strong> 8. intermedius was confirmed<br />
by comparison with the type culture <strong>of</strong> g.<br />
intermedius, which is derived from Emmons and Dodge's<br />
original isolate. In light <strong>of</strong> this study, the<br />
acceptance <strong>of</strong> the genus Pithoascus and the family<br />
Pithoascaceae should be re-examined .<br />
Rogers, J. D., see Glawe, D. 4.<br />
Romaine, C. P., see Wach, M. P., et. al.<br />
Romano, M. A., see Gessner, R. V., et. al.<br />
Ross, I. K., see Kerrigan, R. W.<br />
I. K. Ross, H. T. Choi, and R. L. Wilks. Dept. <strong>of</strong><br />
Biological Sciences, University <strong>of</strong> California, Santa<br />
Barbara, CA 93106. sclerotia and phenoloxidase production<br />
in liquid cultures <strong>of</strong> Coprinus congregatus.<br />
The lack <strong>of</strong> uniformity <strong>of</strong> mycelial balls (pellets)<br />
growing in liquid shake culture is well known, but<br />
usually consists <strong>of</strong> physiological differences among<br />
populations <strong>of</strong> relatively similar cells <strong>of</strong> different<br />
ages and environmental conditions. Such cultures <strong>of</strong><br />
higher fungi are not normally known to undergo differentiation<br />
to form structures composed <strong>of</strong> cells <strong>of</strong><br />
different type and function. On agar cultures, 5.<br />
congregatus may form either mushrooms or sclerotia.<br />
The initial development <strong>of</strong> the early stages <strong>of</strong> both<br />
mushrooms and sclerotia are virtually identical aggregations<br />
<strong>of</strong> hyphae and are probably governed by the<br />
same genetic and biochemical pathways. Phenoloxidases<br />
have been implicated in the first light response <strong>of</strong><br />
- C. congregatus and are now known to be associated with<br />
the very early stages <strong>of</strong> primordium formation. This<br />
oaDer reports the development <strong>of</strong> well-defined scler-