WS_HSE_Catalog_VWR_new - Ward's Science

iomedical technology | activities
87
Southern Blot
Analysis Lab
Activity
Lead Students In a
Hypothetical Paternity
Determination
Introduce students to the
procedures of gel-transfer
hybridization, also known as Southern Blotting. Students use this technique
to detect specific DNA fragments in a simulated paternity determination.
DNA fragments are separated by agarose gel electrophoresis, transferred
to a membrane and then visualized by staining. This experiment is safe
and easy to perform, and does not use human DNA. Includes materials for
five blots. Requires agarose electrophoresis chamber, power supply, and
10–20mL micropipets, available separately.
368955 $115.99
Ward’s Human
Fingerprinting by
PCR Lab Activity
Investigate the Various
Uses of DNA Polymorphism
Identification in Individuals
Certain DNA basepair sequences
vary from person to person; for
example, in one person a particular
basepair sequence may be repeated
ten times, while in another person
the same sequence may be repeated
25 times. Known as polymorphisms,
the repetitions are an identifying
factor in a person’s DNA, and thereby can be used for DNA fingerprinting in
forensics, paternity testing, and disease diagnosis. The basepair sequences can
be separated using electrophoresis and the resulting banding pattern used
to distinguish one person from the next, which students perform with the lab
activity. Using DNA isolated from their own cheek cells, students amplify a variable
DNA region known as a D1S80 locus, electrophorese the DNA, and identify
whether the student is homozygous or heterozygous for the D1S80 locus. The
kit includes enough materials to run 24 PCR reactions, a teacher’s guide, and
student copymaster. A thermal cycler or two water baths, electrophoresis
chamber, power supply, variable volume micropipets, required, are available
separately. Note: Coupon included for perishable materials. Redeem by mail,
fax, phone, or e-mail.
368858 With QUIKView Stain $159.69
Ward’s Paternity
Testing Lab Activity
Determine Familial Relationships
with DNA Fingerprinting
Paternity was once determined by blood
type, based on the concept that a child’s
blood type depends on the blood type
of the parents. Yet blood type can only
exclude possible biological parents, not
definitively determine the identity of the
real parents. With DNA fingerprinting, however,
it is possible to establish familial relationships
with a high degree of certainty.
In this lab activity, students work with a real-life situation,
electrophoresing nonhuman DNA samples, simulating
those of a child, mother, and two possible fathers; compare
the banding patterns; and determine which of the
two men is the most probable match to be the child’s
biological father. The activity can be completed in two 60 minutes sessions and
comes with enough materials to run eight gels, a teacher’s guide, and student
copymaster. An electrophoresis chamber, power supply, and 10 mL micropipets,
required, are available separately. Note: Coupon included for perishable materials.
Redeem by mail, fax, phone, or e-mail.
368551 With QUIKView Stain $101.99
RT-PCR: A Module for the
Molecular Biology of HIV
Replication Lab Activity
Modern Application of Advanced
Science
Use this kit to demonstrate the mode of replication
of HIV. The specific mRNA provided in this experiment
is reverse transcribed to double-stranded
DNA. This DNA product is then amplified by PCR.
The resulting reactions contain reverse transcriptase
and mimic the way HIV replicates. Includes six setups
with instructions. A thermal cycler, horizontal
gel tank, power supply, micropipets with tips, balance,
and water bath, are required, but not included. Note: Coupon included for
perishable materials. Redeem by mail, fax, phone, or e-mail.
368956 $199.69
Ward’s Determining DNA
Concentration and Purity
Lab Activity
Analyze DNA Samples Using UV
Spectrophotometry
Techniques used to quantify DNA or to determine
the purity of a DNA sample are important
precursors to performing further analysis and
validating results. In this activity, students will
first learn to quantify unknown DNA samples
by generating and interpreting a standard curve
based on UV absorbance. They will then isolate DNA from their own cheek
cells and determine the purity of the sample using a ratio of 260/280 nm wavelength.
The kit includes enough materials for eight setups, a teacher’s guide, and
student copymaster. A UV spectrophotometer, microcentrifuge, incubator, and
waterbath, as well as quartz cuvettes and micropipets, all required, are available
separately. Note: Coupon included for perishable materials. Redeem by mail,
fax, phone, or e-mail.
366270 $74.19
Principles of DNA
Sequencing Lab
Activity
Explains Methods Used
For Understanding the
Molecular Structure and
Function of DNA
A dry lab explaining DNA sequencing
and analysis, this activity illustrates
how DNA sequencing is used
to determine the primary structure of DNA. Actual autoradiograms from DNA
sequencing experiments are provided for identification of mutated nucleotides.
Contents can also be viewed using an overhead projector for full class viewing.
For your convenience and safety, this experiment does not contain radioactive
materials, does not require electrophoresis, and does not contain any perishable
reagents or supplies. Highly recommended: visible light box for individual or
student group viewing of autoradiograms. An overhead projector may be used
for entire class viewing. Includes materials for six setups.
368958 $56.65
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