genomewide characterization of host-pathogen interactions by ...

Maren Depke
Results
Kidney Gene Expression Pattern in an in vivo Infection Model
Fig. R.2.9:
Interferon Signaling (modified
from IPA, www.ingenuity.com).
Red color indicates increase of
expression. More intense shade
of color is used for higher
absolute fold change values. A
trend of induction is shown in
yellow.
Similarly, IL-6 receptor (Il6ra), IL-1 receptor (Il1r1, Il1r2) and TNF-α receptor (Tnfrsf1a,
Tnfrsf1b) and their ligands (Il6, Il1a, Il1b, Tnf) were expressed at higher levels in infected tissue
than in control tissue as depicted in the pathway “IL-6 Signaling” (p = 6.88E−11; ratio = 27/93;
Fig. R.2.10). Glycoprotein 130 (Il6st), a signal transducer shared by IL-6 and other cytokines,
exhibited at least a trend of induction. Again, genes for signal transduction molecules and
transcription factors were significantly (Ikbke, Nfkbia, Nfkbia, Nfkbiz, Nfkb2, Stat3, Jun, Fos,
Cebpb) or slightly (Nfkbid, Nfkb1, Traf2, Map4k4, Mapk13, Nras) increased. Signal transduction
was activated in infected tissue, which was demonstrated by the induction of transcriptionally
regulated genes like TSG6 (Tnfaip6), Collagen (Col1a1), alpha-2-macroglobulin (A2m), and IL-6
itself.
Fig. R.2.10: IL-6 Signaling (modified from IPA, www.ingenuity.com).
Direction and magnitude of differential expression are indicated by colors: red – induction; green – repression. More intense shade of
color is used for higher absolute fold change values. A trend of induction is shown in yellow.
86