genomewide characterization of host-pathogen interactions by ...

corticosterone [pg/ml plasma]
corticosterone [pg/ml plasma]
corticosterone [pg/ml plasma]
Maren Depke
R E S U L T S
LIVER GENE EXPRESSION PATTERN IN A MOUSE
PSYCHOLOGICAL STRESS MODEL
The results of the liver gene expression profiling in a mouse psychological stress model have
been published by Depke et al. in 2008 and 2009. All array data are available at the NCBI’s Gene
Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) database and are accessible
through GEO Series accession no. GSE11126. Furthermore, lists of differentially expressed genes
are included as supplemental data (Depke et al. 2008) at the journal’s homepage
(http://endo.endojournals.org). Stress and infection experiments, physiological measurements,
and histological analyses were performed by Cornelia Kiank. Her data are cited here because they
are essential for the understanding of physiological events and potential dysregulations during
acute and chronic stress in mice.
Repeated stress-induced cachexia accompanied by hypercortisolism, hyperleptinemia, and
hypothyroidism [Cornelia Kiank]
Repeated psychological stress caused a severe loss of body mass in BALB/c mice while mean
food intake and water consumption were unaltered during 4.5 days stress exposure. Food intake
was 95.1 ± 19.9 g/cage in the repeatedly stressed vs. 91.9 ± 17.4 g/cage in the nonstressed
groups, and water consumption was 250 ± 40 ml/cage in stressed vs. 240 ± 40 ml/cage in the
control mice (three independent experiments with nine mice per cage). As the food and water
intake was consistently found to be normal, the question arose whether the severe loss of body
mass after repeated stress exposure was dependent on hormonal changes. Repeatedly stressed
mice showed increased corticosterone concentrations in the peripheral blood (Fig. R.1.1 A) along
with a hypertrophy of the adrenal cortex with decreased size of lipid storage vesicles in the
glucocorticoid-producing zona fasciculata (Fig. R.1.1 B, C).
A
A
A750 *
A
750 *
750 *
B
B
B
B
Fig. R.1.1: Repeated stress-induced activation of the HPA axis in BALB/c
mice.
A. Increased plasma corticosterone levels in repeatedly stressed mice
(black box plot) compared with nonstressed mice (white box plot) (n = 9
mice per group).
B, C. Hypertrophy of the zona fasciculata of the adrenal cortex (white line)
in repeatedly stressed mice (B) compared with nonstressed controls (C)
(HE staining magnification, x100); each picture is representative for nine
mice per group. *, p < 0.05 Mann-Whitney U test; data reproduced in at
least three independent experiments.
500
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500
250
250
250
0
0
0
CC
C
C
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